Isolation and purification of neuraminidase from influenza vaccine and reference substance preparation
10.3760/cma.j.cn112309-20210301-00071
- VernacularTitle:流感疫苗神经氨酸酶的分离纯化方法建立及其参考品制备
- Author:
Tao DENG
1
;
Jiayou ZHANG
;
Xiaoming YANG
Author Information
1. 武汉生物制品研究所病毒性疫苗研究二室 430207
- Keywords:
Neuraminidase;
Influenza vaccine;
Isolation and purification;
Reference substance
- From:
Chinese Journal of Microbiology and Immunology
2021;41(11):875-879
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for isolation and purification of neuraminidase from influenza vaccine and to prepare reference substance for quantitative detection of neuraminidase.Methods:Functional magnetic particles with specific affinity for neuraminidase were prepared. The method for separation and purification of neuraminidase was established based on the magnetic particles. The separation and purification conditions were optimized. The purity of neuraminidase was analyzed and the specificity was verified. The enzyme activity was determined and the protein was quantified.Results:The functional magnetic particles modified with 4-aminophenanthroline were successfully prepared and the method for isolation and purification of neuraminidase based on the magnetic particles was established. The purity of neuraminidase was 98.7%. The concentrations of neuraminidase isolated and purified from the monovalent stock solution of H1N1, H3N2, B/Victoria and B/Yamagate vaccines were 71.50, 100.58, 64.11 and 37.68 μg/ml, respectively, and the enzyme activity remained.Conclusions:The method for isolation and purification of influenza virus neuraminidase was established and the corresponding reference substance was prepared.
