Engineered polymer nanoparticles incorporating L-amino acid groups as affinity reagents for fibrinogen
- Author:
Zhu YONGYAN
1
;
Liu RUIXUAN
;
Wu DENGYU
;
Yu QIANQIAN
;
J.Shea KENNETH
;
Zhu QUANHONG
Author Information
1. School of Traditional Chinese Medicine,Southern Medical University,Guangzhou,510515,China;Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics,Guangzhou,510515,China;Guangdong Provincial Engineering Laboratory of Chinese Medicine Preparation Technology,Guangzhou,510515,China
- Keywords:
Synthetic polymer nanoparticles;
Amino-acid monomers;
Arginine;
Fibrinogen;
Affinity reagent;
Protein interaction
- From:
Journal of Pharmaceutical Analysis
2021;11(5):596-602
- CountryChina
- Language:Chinese
-
Abstract:
Synthetic polymer hydrogel nanoparticles(NPs)were developed to function as abiotic affinity reagents for fibrinogen.These NPs were made using both temperature-sensitive N-isopropyl acrylamide(NIPAm)and L-amino acid monomers.Five kinds of L-amino acids were acryloylated to obtain functional mono-mers:L-phenylalanine(Phe)and L-leucine(Leu)with hydrophobic side chains,L-glutamic acid(Glu)with negative charges,and L-lysine(Lys)and L-arginine(Arg)with positive charges.After incubating the NPs with fibrinogen,y-globulin,and human serum albumin(HSA)respectively,the NPs that incorporated N-acryloyl-Arg monomers(AArg@NPs)showed the strongest and most specific binding affinity to fibrin-ogen,when compared with y-globulin and HSA.Additionally,the fibrinogen-AArg binding model had the best docking scores,and this may be due to the interaction of positively charged AArg@NPs and the negatively charged fibrinogen D domain and the hydrophobic interaction between them.The specific adsorption of AArg@NPs to fibrinogen was also confirmed by the immunoprecipitation assay,as the AArg@NPs selectively trapped the fibrinogen from a human plasma protein mixture.AArg@NPs had a strong selectivity for,and specificity to,fibrinogen and may be developed as a potential human fibrinogen-specific affinity reagent.
