Correlation between T follicular helper cells and eosinophilic gastroenteritis in children
10.3760/cma.j.cn101070-20200806-01303
- VernacularTitle:滤泡辅助性T淋巴细胞与儿童嗜酸性粒细胞性胃肠炎的相关性研究
- Author:
Hongmei GUO
1
;
Jinxia FAN
;
Lanfang ZHANG
;
Zhifeng LIU
Author Information
1. 南京医科大学附属儿童医院消化科 210008
- Keywords:
Eosinophilic gastroenteritis;
T follicular helper cells;
Inducible costimulatory molecule;
Programmed death 1;
Immunoglobulin E;
Flow cytometry
- From:
Chinese Journal of Applied Clinical Pediatrics
2021;36(19):1480-1483
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role and clinical significance of T follicular helper (Tfh) cells in the pathogenesis of eosinophilic gastroenteritis (EG) in children.Methods:A total of 17 children diagnosed with EG in the Department of Gastroenterology, Children′s Hospital of Nanjing medical University from October 2018 to January 2020 were recruited as EG group.During the same period, 15 children diagnosed with colon polyps were included as control group.Flow cytometry was used to detect Tfh cells and their functional molecules, including inducible costimulatory molecules (ICOS) and programmed cell death protein 1 (PD-1) in peripheral blood of the 2 groups.Results:The median (interquartile range) of Tfh cell frequency in peripheral blood of children with EG group was 7.3 (2.6)%, which was significantly higher than that of controls 2.8 (1.4)% ( P<0.05). There were significant differences in the median (interquartile range) of ICOS [1.5 (1.3)% vs.0.1 (0.2)%] and PD-1 expressions [1.8 (3.2)% vs.0.7 (0.6)%] on Tfh cells between children with EG group and control group (all P<0.05). The frequency of Tfh cells in the peripheral blood of children with EG was positively correlated with the expressions of ICOS ( r=0.746, P<0.05) and PD-1 ( r=0.893, P<0.05), and immunoglobulin E (IgE) level ( r=0.587, P<0.05). Conclusions:The frequency of Tfh cells in peripheral blood of children with EG significantly increases, which are proliferative and overexpressed with ICOS and PD-1.Moreover, the frequency of Tfh cells of EG patients is positively correlated with the level of IgE.The abnormal expression of Tfh cells may play a promoting role in the mechanism of EG.
