miR-187-5p alleviates osteoporosis through inhibiting activity of osteoclast via RANKL/NFκB
10.3760/cma.j.cn.115807-20210225-00060
- VernacularTitle:miR-187-5p通过RANKL/NFκB信号通路抑制骨质疏松症中的破骨细胞活性
- Author:
Yuanpeng GAO
1
;
Min ZHANG
;
Bin CHEN
;
Junjun SHI
;
Yuze WANG
;
Dong WANG
;
Yuan LI
Author Information
1. 山西医科大学第二医院骨科，太原 030000
- Keywords:
miR-187-5p;
Osteoporosis;
RANKL/NFκB;
Osteoclast
- From:
Chinese Journal of Endocrine Surgery
2021;15(4):394-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of microRNA-187-5p (miR-187-5p) on osteoporosis (OP) as well as the related molecular mechanism.Methods:The level of miR-187-5p and tartrate-resistant acid phosphatase (TRAP, the osteoclast marker) was quantitatively detected in TRANKL-induced OP group and control (con) group by reverse transcription-quantitative PCR (RT-qPCR) ; Cell counting kit 8 (CCK8) was used to measure the viability of murine macrophage cell line (RAW264.7) ; Western blot was used to detect the expression of p65 and TNFα. Moreover, the dual luciferase reporter assays was applied to detect the interaction between miR-187-5p and p65.Results:The osteoclast proliferation determined by CCK8 and the mRNA level of TRAP detected by RT-qPCR demonstrated that the growth of osteoclast was inhibited in OP group compared with that in con group ( P=0.008; P=0.017) , which reversed by miR-187-5p mimic stimulation ( P=0.023; P=0.037) . The miR-187-5p was lowly expressed in OP group compared with that in con group ( P=0.031) ,which was increased by miR-187-5p mimic treatment compared with miR-187-5p NC treatment ( P=0.041) . Moreover, western blot indicated that the protein level of p65 and TNFα in OP group were up-regulated compared with that in con group ( P=0.034; P=0.024) , which restored by the miR-187-5p mimic co-incubation ( P=0.028; P=0.036) . Moreover, the RT-qPCR indicated that the mRNA level of p65 in OP group was also increased compared with that in con group ( P=0.039) , and the miR-187-5p mimic co-incubation decreased the mRNA level of p65, compared with miR-187-5p NC group ( P=0.025) . The dual luciferase reporter assays indicated that there was an interaction between miR-187-5p and p65. Conclusion:miR-187-5p alleviates OP through inhibiting the activity of osteoclast via RANKL/NFκB signaling
