Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial Cystitis.
10.5213/inj.2015.19.3.158
- Author:
Ki Hak SONG
1
;
Chang Shik YOUN
;
Chung Lyul LEE
;
Seung Woo YANG
;
Young Seop CHANG
;
Seoung Woo JEONG
;
Chong Koo SUL
Author Information
1. Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea. snowman@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Neuregulin-1;
Receptor, ErbB-2;
Cystitis, Interstitial;
Rats
- MeSH:
Animals;
Blotting, Western;
Cyclophosphamide;
Cystitis, Interstitial*;
Neuregulin-1*;
Protein-Tyrosine Kinases*;
Rats*;
Rats, Sprague-Dawley;
Receptor, erbB-2;
Regeneration;
RNA, Messenger;
Tyrosine*;
Urinary Bladder*;
Urinary Bladder, Overactive
- From:International Neurourology Journal
2015;19(3):158-163
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The aim of this study was to evaluate changes in expressions of neuregulin (NRG)1 and erbB2 tyrosine kinase (ErbB2) in bladders of rats with cyclophosphamide (CYP)-induced interstitial cystitis (IC). METHODS: Twenty-four Sprague-Dawley rats were divided into the IC group (n=16) and the control group (n=8). After inducing IC with intraperitoneal CYP injection, expressions of NRG1 and ErbB2 were analyzed using western blotting and reverse transcriptase-polymerase chain reaction. RESULTS: In Western blotting, relative intensities and distributions of both NRG1 and ErbB2 were approximately 1.5- and 3.2-fold higher, respectively, in the IC group than in the control group (mean+/-standard deviation: 1.42+/-0.09 vs. 0.93+/-0.15 and 0.93+/-0.16 vs. 0.29+/-0.08, P<0.05). In the rat bladder samples, mRNA expression levels of NRG1 and ErbB2 were higher in the IC group than in the control group (P<0.05). CONCLUSIONS: Our study has demonstrated significant changes in mRNA expression and immunoreactivity of NRG1 and ErbB2 receptors in the urinary bladder after CYP-induced IC. These results suggest that the up-regulated NRG1 may play a role in inducing an overactive bladder and promoting regeneration in the inflammatory bladder with CYP-induced IC.
