Study on alkaloid constituents of Stephania tetrandra S. Moore
10.16438/j.0513-4870.2021-1170
- VernacularTitle:粉防己生物碱成分研究
- Author:
Da-hai HE
1
;
Jun LIU
2
;
Dong-mei FANG
3
;
Xiao-ling WANG
1
;
Li-mei LI
1
Author Information
1. College of Pharmacy, Southwest Minzu University, Chengdu 610041, China
2. College of Chemistry and Environment, Southwest Minzu University, Chengdu 610041, China
3. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
- Publication Type:Research Article
- Keywords:
italic>Stephania tetrandra;
alkaloid;
X-ray diffraction;
tetrandraside A
- From:
Acta Pharmaceutica Sinica
2021;56(12):3503-3510
- CountryChina
- Language:Chinese
-
Abstract:
Fifteen alkaloid compounds were isolated from the 70% aqueous alcohol extract of Stephania tetrandra S. Moore by silica gel, reversed phase silica gel, Sephadex LH-20 column chromatography and semi-preparative high performance liquid chromatography. They were identified as tetrandraside A (1), (Z)-N-formyl-nornuciferin (2), (E)-N-formyl-nornuciferin (3), salutaridine (4), salutaridine N-oxide (5), (E)-3-(4-hydroxy-3-methoxyphenyl)-N-[2-(4-hydroxy-3-methoxyphenyl)ethyl]-2 propenamide (6), dauriporphine (7), sinomenine (8), liriodenine (9), α-magnoflorine (10), (1S)-4'-β-glucosylcoclaurine (11), tetrandrine (12), fangchinoline (13), tetrandrine 2'-β-oxide (14), and tetrandrine 2'-α-oxide (15), respectively, by MS, NMR and single crystal diffraction. Among them, compound 1 is a new alkaloid glycoside. Compounds 2-11 were obtained from this plant for the first time. These compounds showed obvious cytotoxic activity against drug-resistant lung cancer cell line H1299, and compound 9 had the best activity, with an IC50 of 5.38 μmol·L-1.
