Analysis of Chemical Constituents in Euodiae Fructus by UPLC-Q-TOF-MS/MS
10.13422/j.cnki.syfjx.20202051
- VernacularTitle:基于UPLC-Q-TOF-MS/MS技术的吴茱萸化学成分分析
- Author:
Xiao-mei ZHAO
1
;
Yu-xin CHENG
2
;
Cai-xia LIANG
2
;
Jie GUO
2
;
Xiao-qian LIU
2
;
Wei-hong FENG
2
;
Zheng-bao ZHAO
3
;
Li-hua YAN
2
;
Zhi-min WANG
2
Author Information
1. Shanxi University of Chinese Medicine，Jinzhong 030619，China
2. National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines， Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
3. Shanxi Medical University，Taiyuan 030001，China
- Publication Type:Research Article
- Keywords:
Euodiae Fructus;
ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF-MS/MS）;
alkaloids;
flavonoids;
limonoids;
phenolic acids;
organic acids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(6):113-126
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the chemical constituents in Euodiae Fructus by ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF-MS/MS）. Method:The chromatographic separation was performed on an ACQUITY UPLC BEH C18 column （2.1 mm×100 mm， 1.7 μm） with acetonitrile （A）-0.1% formic acid aqueous solution （B） as mobile phase （0-3 min， 6%A； 3-4 min， 6%-10%A； 4-7 min， 10%-12%A； 7-8 min， 12%-14%A； 8-13 min， 14%-15%A； 13-15 min， 15%-20%A； 15-18 min， 20%-30%A； 18-21 min， 30%-49%A； 21-25 min， 49%-51%A； 25-27 min， 51%-73%A； 27-30 min， 73%-80%A； 30-31 min， 80%-100%A； 31-32 min， 100%A） for gradient elution. The column temperature was 35 ℃， and the flow rate was 0.4 mL·min-1. Mass spectrometry was performed using an electrospray ionization and data were collected in positive and negative ion modes， and the detection range was m/z 100-1 200. The chemical constituents in Euodiae Fructus were identified rapidly and comprehensively based on the accurate relative molecular mass and combined with literature data and reference substances. Result:A total of 92 chemical constituents were speculatively identified from the 70% methanol extract of Euodiae Fructus， including 39 alkaloids， 19 flavonoids， 12 limonoids， 20 phenolic acids and 2 organic acids. Among them， 26 compounds were confirmed by the reference substances. Conclusion:The compound types of Euodiae Fructus are multiple and quite different in polarity. The chemical compositions of Euodiae Fructus from different regions and species are similar. The established method is rapid and accurate， with which the chemical compositions of Euodiae Fructus have been identified comprehensively. Therefore， this study provides an experimental reference for further clarifying active and toxic constituents of Euodiae Fructus.
