Effect of Weiwei Tongtiao Decoction on Expression of IKKβ, Bcl-2 in CAG Precancerous Lesion Rats
10.13422/j.cnki.syfjx.20202329
- VernacularTitle:萎胃通调汤对大鼠慢性萎缩性胃炎癌前病变及IKKβ，Bcl-2表达的影响
- Author:
Shao-wei YOU
1
;
Xu YI
1
;
Qi ZHAO
1
;
Wen-su WANG
1
;
Ling YANG
2
;
Ping-zhen TONG
1
;
Xue-yong WANG
3
;
Ya-mei ZHAN
4
Author Information
1. The 2nd Hospital Affiliated Guizhou University of Traditional Chinese Medicine，Guiyang 550003，China
2. People′s Hospital of Changshou Chongqing，Chongqing 401220，China
3. Beijing University of Chinese Medicine，Beijing 102488，China
4. Guizhou Provincial People′s Hospital，Guiyang 550002，ChinaB
- Publication Type:Research Article
- Keywords:
Weiwei Tongtiao decoction;
chronic atrophic gastritis（CAG）;
precancerous lesion;
inhibitor κB kinase β（IKKβ）;
B-cell lymphoma-2（Bcl-2）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(6):55-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Weiwei Tongtiao decoction on gastric mucosal pathology and the expression level of inhibitor kappa B kinase β（IKKβ） and B-cell lymphoma-2（Bcl-2）in rats with chronic atrophic gastritis （CAG） precancerous lesion. Method:SD rats were randomly divided into normal group， model group， positive drug Weifuchun group， Weiwei Tongtiao decoction high， medium and low dose treatment groups. The rat model of CAG precancerous lesion was prepared by N-methyl-N'-nitro-N-nitrosoguanidine （MNNG）compound modeling method. weiwei Tongtiao decoction high， medium and low dose treatment groups received intragastric administration of 24， 12， 6 g·kg-1 Weiwei Tongtiao decoction respectively， while Weifuchun group received 0.45 g·kg-1 Weifuchun suspension， once per day for 12 weeks. The pathological changes of gastric mucosa of rats were observed by hematoxylin-eosin（HE）staining， and the mRNA and protein levels of IKKβ and Bcl-2 in gastric mucosa of rats were detected by Real-time quantitative polymerase chain reaction （Real-time PCR）， immunohistochemistry（IHC）and Western blot. Result:Compared with the normal group， 100% inherent gland atrophy， mild to severe intestinal metaplasia， and 25% low-grade intraepithelial neoplasia were observed under microscope in model group. All Weifuchun group and Weiwei Tongtiao decoction groups could improve the atrophy of gastric glands， moderate to severe intestinal metaplasia and pathological injury of low-grade intraepithelial neoplasia， especially at high dose group. Compared with the normal group， IKKβ， Bcl-2 mRNA and protein expressions in the gastric mucosa of the model group were up-regulated （P<0.01）. Compared with the model group， the mRNA and protein expressions of IKKβ and Bcl-2 in gastric mucosa of rats in the Weifuchun group and the Weiwei Tongtiao decoction high， medium and low dose groups were down-regulated （P<0.05，P<0.01）， showing a dose-dependent relationship， and such levels in the Weiwei Tongtiao decoction high-dose intervention group were similar to those in normal group. Conclusion:Weiwei Tongtiao decoction can improve and even reverse gastric mucosa with CAG precancerous lesions in rats， and its intervention mechanism may be related to down-regulating the expressions of IKKβ and Bcl-2 in gastric mucosa.
