Experimental Study of Yuxuebi Tablets by Inhibiting Peripheral Inflammation to Relieve Hyperalgesia and Foot Swelling in Mice with Chronic Inflammatory Pain
10.13422/j.cnki.syfjx.20210806
- VernacularTitle:瘀血痹片通过抑制外周炎症缓解慢性炎性痛小鼠痛觉过敏及足肿胀
- Author:
Jia-hao LI
1
;
Cong-min TIAN
2
;
Hong-yu CHI
2
;
Guo-xin ZHANG
2
;
Jun YANG
2
;
Chun-yan ZHU
2
;
Na LIN
1
Author Information
1. School of Pharmacy，Fujian University of Traditional Chinese Medicine，Fuzhou 350108，China
2. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences， Beijing 100700，China
- Publication Type:Research Article
- Keywords:
Yuxuebi tablets;
chronic inflammatory pain;
anti-inflammatory;
hyperalgesia;
swelling;
inflammatory factors
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(10):31-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Yuxuebi tablets on hyperalgesia and foot swelling in mice with chronic inflammatory pain， and to explore the preliminary mechanism of action. Method:A mouse model of chronic inflammatory pain was established with left plantar injection of complete Freund's adjuvant （CFA）. The mice were divided into model group， positive drug ibuprofen group （91 mg·kg-1）， Yuxuebi tablets low， medium and high dose groups （55， 110， 220 mg·kg-1），with the sham operation group as the control. After successful modeling， the daily dose was divided into two doses in the morning and evening by gavage to give Yuxuebi tablets or ibuprofen to the stomach for a total of 19 days. On the 18th day after the administration， the thermal pain threshold was detected by the hot plate method. On the 19th day， the standard Von Frey fiber needle was used to detect the mechanical pain threshold of the mice， and the degree of foot swelling was scored and photographed. The liquid-phase suspension chip technology was used to quantitatively analyze 36 classic broad-spectrum inflammation-related factors like inflammatory factors and receptors. Bioinformatics were used to screen core targets and perform enzymelinked immunosorbent assay （ELISA） detection. Result:Compared with the sham operation group， the mechanical pain threshold and foot swelling score of the model froup significantly increased （P<0.01）， the latent time of heat sensitivity significantly decreased（P<0.01）， the expressions of 30 inflammatory factors in the foot increased（P<0.05）. Compared with the model group， the high dose of Yuxuebi tablets significantly reduced the mechanical pain threshold and foot swelling score of mice with chronic inflammatory pain（P<0.01）， significantly increased the latent time of heat sensitivity（P<0.05）， and reduced the expressions of 30 inflammatory factors in the foot（P<0.05）， among which tumor necrosis factor-α （TNF-α）， interleukin-6 （IL-6）， interleukin-17A （IL-17A）， and C-C chemokine ligand 2 （CCL2） were the core targets screened out， and the expressions of TNF-α， IL-17A， and CCL2 significantly decreased （P<0.01）. Conclusion:Yuxuebi tablets can relieve hyperalgesia and foot swelling in mice with chronic inflammatory pain， and its mechanism may be related to the inhibition of the expressions of peripheral inflammatory factors such as TNF-α， IL-17A， and CCL2 .
