Comparison of Relative Molecular Weight Distribution and Immune Activity of Polysaccharides in Polygonati Rhizoma Before and After Processing
10.13422/j.cnki.syfjx.20210446
- VernacularTitle:黄精炮制前后多糖的相对分子质量分布和免疫活性比较
- Author:
Xiao-ying WAN
1
;
Zhen-li LIU
1
;
Zhi-qian SONG
1
;
Zhang-chi NING
1
;
Shi-tao PENG
1
;
Dong-rui LIANG
1
;
Chun WANG
1
Author Information
1. Institute of Basic Theory of Chinese Medicine， China Academy of Chinese Medical Sciences，Beijing 100700，China
- Publication Type:Research Article
- Keywords:
processing with wine;
Polygonati Rhizoma;
polysaccharides;
relative molecular weight;
macrophages;
immune function;
inflammatory response
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(15):83-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the relative molecular weight and distribution of polysaccharides in Polygonati Rhizoma （PR） before and after processing， as well as the effects of different polysaccharide fractions on immune function and inflammatory response of mouse peritoneal macrophages. Method:High-performance gel permeation chromatography （HPGPC） was used to determine the relative molecular weight and distribution of polysaccharides in PR （named SC） and polysaccharides in PR processed with wine （named JC）， and polysaccharide fractions with different relative molecular weights were obtained by dialysis. Different polysaccharide fractions were applied to mouse peritoneal macrophages， which was normal or induced by lipopolysaccharide （LPS）. Cell counting kit-8 （CCK-8） method was used to select the optimal administration concentration. Enzyme linked immunosorbent assay （ELISA） was used to determine the concentrations of tumor necrosis factor-α （TNF-α） and interleukin-1β （IL-1β） in the cell supernatant. The Griess method was used to detect the concentration of nitric oxide （NO）. Result:SC and JC could be divided into four parts according to relative molecular weight and its distribution range， including part Ⅰ（14 800-2 273 kDa）， part Ⅱ（2 148-296 kDa）， part Ⅲ（12-1 kDa） and part Ⅳ（818-362 Da）. Based on the differences of part Ⅰ and part Ⅲ after processing， the SC and JC were divided into two-part according to the weight-average relative molecular weight （MW）. For normal mouse peritoneal macrophages， JC could significantly promote the secretion of TNF-α （P<0.01）， while SC had no significant effect. Four polysaccharide fractions， named SD （SC fraction with MW>50 kDa）， JD （JC fraction with MW>50 kDa）， SX （SC fraction with MW<50 kDa） and JX （JC fraction with MW<50 kDa）， also could significantly promote the secretion of TNF-α （P<0.01）， but only JX could significantly promote the secretion of NO （P<0.05）. In addition， the effect of JX group stimulated secretion of TNF-α was better than the JD group （P<0.01）. For the LPS-induced macrophage model， JC and SC group could significantly inhibit the secretion of TNF-α and IL-1β （P<0.01）， and the effect of JC was stronger. To compare different polysaccharide fractions， the impact of JX on inhibiting the secretion of TNF-α and IL-1β was significantly stronger than JD （P<0.01）， and SX inhibited the secretion of TNF-α was significantly stronger than SD （P<0.01）. Conclusion:The relative molecular weight and distribution of polysaccharides in PR before and after processing have changed. JC and SC improve the immune regulation mainly by inhibiting the inflammatory response， the fraction of MW<50 kDa is the main effective part， and the effect of PR polysaccharides in inhibiting the inflammatory is enhanced after processing with wine.
