Analysis of Chemical Constituents in Microctis Folium by UPLC-Q-TOF-MS/MS
10.13422/j.cnki.syfjx.20201948
- VernacularTitle:基于UPLC-Q-TOF-MS/MS技术的布渣叶化学成分分析
- Author:
Guan-lin XIAO
1
;
Jie-yi JIANG
1
;
Su-mei LI
1
;
Wei-tao CHEN
1
;
Zhao CHEN
1
;
Jing-nian ZHANG
1
;
Xiao-li BI
1
Author Information
1. Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine （TCM），Guangdong Province Engineering Technology Research Institute of TCM，Guangzhou 510095，China
- Publication Type:Research Article
- Keywords:
Microctis Folium;
ultra performance liquid chromatography-quadrupole-time-of-flight high resolution mass spectrometry （UPLC-Q-TOF-MS/MS）;
chemical constituents;
flavone C-glycosides;
flavonol oxyglycosides;
proanthocyanidins;
organic acids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(3):138-148
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the chemical constituents in Microctis Folium by ultra performance liquid chromatography-quadrupole-time-of-flight high resolution mass spectrometry （UPLC-Q-TOF-MS/MS）. Method:Waters CORTECS UPLC C18 column （2.1 mm×150 mm， 1.6 μm） was used for chromatographic separation with the mobile phase of methanol （A） -0.1% formic acid solution （B） for gradient elution （0-4 min， 14%-30%A； 4-16 min， 30%-58%A； 16-25 min， 58%-78%A； 25-25.1 min， 78%-98%A； 25.1-29 min， 98%A）， the flow rate was 0.25 mL· min-1， the injection volume was 1 μL. The electrospray ionization （ESI） was adopted for determining the chromatographic effluent under positive and negative ion modes， the main chromatographic peaks were assigned and distinguished by Q-TOF， and the scanning range was m/z 100-1 500. Result:A total of 31 chemical constituents in Microctis Folium were identified by confirmation of reference substances， literature comparison and high resolution mass spectrometry data analysis. The chemical constituent cluster was composed of 28 flavonoids （9 flavone C-glycosides， 10 flavonols and their glycosides， 8 proanthocyanidins， 1 xanthone） and 3 organic acids （caffeic acid， p-coumaric acid， ferulic acid）. Conclusion:UPLC-Q-TOF-MS/MS technique provides a simple， rapid and accurate method for the identification of chemical constituents in Microctis Folium. Flavone C-glycosides， flavonol oxyglycosides and proanthocyanidins are the main chemical constituents. The 7 proanthocyanidins are reported for the first time in this herb. In conclusion， the chemical profile of Microctis Folium is characterized and the findings are meaningful for the in-depth quality assessment and material basis clarification of Microctis Folium.
