Discussion on Effect of Zuoguiwan on Skin Development of Kidney-deficient Rats Based on Autophagy
10.13422/j.cnki.syfjx.20210502
- VernacularTitle:基于细胞自噬探讨左归丸对肾虚仔鼠皮肤发育的影响
- Author:
Ji-gang CAO
1
;
Ying LIU
1
;
Hong-ling SANG
1
;
Min ZHAO
1
Author Information
1. Hubei University of Chinese Medicine， Wuhan 430065， China
- Publication Type:Research Article
- Keywords:
autophagy;
Zuoguiwan;
skin;
development;
kidney deficient;
offspring rat
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(8):1-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect and mechanism of Zuoguiwan on the autophagy of cells in the skin during the development of congenital kidney-deficient mice， and to explore the application value of kidney tonifying and essence filling method in abnormal growth and development. Method:36 rats with a 2∶1 ratio of male to female were paired， and the pregnant rats were stimulated by combined stress method to build a model of congenital kidney-deficient rats. According to different treatment methods， the pregnant rats were divided into the control group， the kidney-deficient group， and the Zuoguiwan low and high dose groups （2， 8 g·kg-1）. The control group was given normal saline without modeling. Kidney deficiency group was modeled before intragastric administration of normal saline. Zuoguiwan small dose and high dose groups received intragastric administration of Zuoguiwan suspension. 21 days after the birth of mice， the back skin was taken to observe the skin microstructure and morphology of the mice by HE staining and detect the thickness of dermis and epidermis. The gene and protein expression levels of Wingless3a and β-catenin in the skin of neonatal rats were measured by Western blot and Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR）. Double immunofluorescence assay was used to detect the skin autophagy indicators Microtubule-associated protein light chain 3 （LC3）and Sequestosome 1 （SQSTM1-p62）. Result:In the control group， the skin layers were clear and the structure was normal， in the kidney deficiency group， the collagen arrangement in the dermis was loose， the skin composition in Zuoguiwan low and high dose groups was basically normal. Compared with the control group， the epidermal layer of the kidney deficiency group was thickened and the dermis was thinned （P<0.05）. Compared with the kidney deficiency group， the thickness changes of epidermal layer and dermis layer were significantly restored in Zuoguiwan low and high dose groups （P<0.05）. Compared with the control group， the expression of Wnt3a and β-catenin protein and gene in the kidney deficiency group significantly decreased （P<0.05）. Compared with the kidney deficiency group， Wnt3a and β-catenin protein and gene expression increased in Zuoguiwan low and high dose groups （P<0.05）. At the same time， immunofluorescence assay showed that compared with the control group， the expression of LC3 was up-regulated and the expression of SQSTM1-p62 was down-regulated in the kidney deficiency group， while Zuoguiwan could reverse such abnormal expression （P<0.05）. Conclusion:Zuoguiwan can down-regulate the autophagy level of skin cells and improve the abnormal skin development of congenital renal deficiency by increasing the expression of Wnt3a and β-catenin protein and gene in the skin of neonatal rats.
