Identification and Expression Analysis of TIFY Transcription Factor in Eucommia ulmoides
10.13422/j.cnki.syfjx.20211211
- VernacularTitle:杜仲TIFY转录因子鉴定与表达分析
- Author:
Jun LIU
1
;
Yu-long CHEN
1
;
Yan LIU
1
;
Fu-chun SI
1
Author Information
1. Henan Key Laboratory of Traditional Chinese Medicine Prescription and Syndrome Signaling，Academy of Chinese Medicine，Henan University of Chinese Medicine，Zhengzhou 450046，China
- Publication Type:Research Article
- Keywords:
Eucommia ulmoides;
TIFY transcription factor;
phylogenetic evolution;
expression analysis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(19):165-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the TIFY gene family in Eucommia ulmoides and analyze its expression from the whole genome level to lay the foundation for further study on EuTIFYs gene function. Method:Based on the E. ulmoides genome database，the TIFY gene family was identified through bioinformatics analysis tools such as National Center for Biotechnology Information（NCBI），MEME，PlantCare，Expert Protein Analysis System（ExPASy），and TBtools. Physicochemical properties，phylogenetic evolution，gene structure，cis-acting elements of the promoters and their expression patterns in leaf development and gum formation of the gene family were systematically analyzed. Result:In this study，fourteen EuTIFY genes （EuTIFY1-EuTIFY14） were identified in the E. ulmoides genome. The EuTIFYs were composed of 312-1 074 bp nucleotides encoding 102-357 amino acid residues，with isoelectric points of 4.99-10.06 and molecular weight in the range of 10.8-39.14 kDa. According to putative subcellular localization，proteins，which were mainly hydrophilic proteins，localized in the nucleus. The 14 EuTIFYs were unevenly distributed on 13 chromosomes. EuTIFY gene family was divided into four subfamilies： TIFY，JAZ，ZML，and PPD，which contained three，four，five，and two members respectively. The promoters of EuTIFYs contained multiple photoperiodic and abiotic stress-responsive cis-acting elements，which were involved in plant growth and abiotic stress regulation. Expression pattern analysis showed that EuTIFYs exhibited different expression levels in different development stages of E. ulmoides leaves and multiple interactions，and most of the genes were highly expressed in the early stage of leaf development and positively regulated the formation of E. ulmoides gum. Conclusion:Fourteen EuTIFYs were identified from the whole genome of E. ulmoides，and their structural characteristics and expression patterns were analyzed by bioinformatics. The findings of this study are expected to provide references for further research on the function of EuTIFYs.
