Buyang Huanwutang Alleviated Oxidative Stress Following Cerebral Ischemia/Reperfusion in Rats by Formyl Peptide Receptor 2
10.13422/j.cnki.syfjx.20211838
- VernacularTitle:补阳还五汤通过甲酰肽受体2减轻大鼠脑缺血再灌注后氧化应激损伤
- Author:
Fang-hua WU
1
;
Yu-lian LIU
1
;
Yu-rong GAO
1
;
Kai-ling YANG
1
;
Wei LIU
1
Author Information
1. School of Basic Medical Sciences， Guangzhou University of Chinese Medicine， Guangzhou 510006， China
- Publication Type:Research Article
- Keywords:
Buyang Huanwutang;
cerebral ischemia/reperfusion;
oxidative stress;
apoptosis;
formyl peptide receptor 2（FPR2）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(18):9-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of formyl peptide receptor 2 （FPR2） in the inhibitory effects of Buyang Huanwutang （BYHWT） on the oxidative stress and its protective effects on cerebral ischemia-reperfusion in rats. Method:Forty-eight male SD rats were randomly divided into sham group， model group， BYHWT group and BYHWT combined with FPR2 inhibitor （Boc-2） group. In the sham group， only the vessels were isolated. In other groups， the middle cerebral artery occlusion （MCAO） model was constructed using the modified Longa method and reperfused after 2 h of ischemia. BYHWT （16 g·kg-1） was given by gavaged twice daily after reperfusion in BYHWT group and BYHWT+Boc-2 group. Boc-2 （0.4 mg·kg-1） was injected intraperitoneally 30 min before surgery. Equal volume of saline were given instead in sham and model group. After 24 h of reperfusion， Fluoro-Jade C （FJC） staining was performed to observe the changes in the number of FJC-positive cells. Western blot was performed to detect the expression of apoptosis-related B-cell lymphoma-2 (Bcl-2)， Bcl-2 associated X (Bax)， and cleaved aspartic acid cysteine proteolytic enzyme-3（Caspase-3）. Besides， superoxide dismutase （SOD）， malondialdehyde （MDA）， glutathione （GSH）， and nitric oxide （NO） was measured. The mean fluorescence intensity of nicotinamide adenine dinucleotide phosphate Ⅱ（NADPH） oxidase 2 （NOX2） was examined by immunofluorescence. Result:Compared with sham group， the model group showed increased number of FJC-positive cells （P<0.01）， decreased Bcl-2 expression （P<0.01）， increased Bax and cleaved Caspase-3 expression （P<0.01）， increased NO and MDA content （P<0.05，P<0.01）， decreased GSH and SOD activities （P<0.05，P<0.01）， and increased NOX2 expression （P<0.01）. Compared with model group， there were decreased FJC-positive cells （P<0.01）， up-regulated Bcl-2 expression （P<0.01） with down-regulated cleaved Caspase-3 and Bax （P<0.05，P<0.01）， decreased NO and MDA （P<0.05，P<0.01） with increased GSH and SOD （P<0.01）， and decreased NOX2 expression （P<0.01） in the BYHWT group. All the above effects were partially blocked by Boc-2. Conclusion:BYHWT can reduce oxidative stress injury and inhibit apoptosis in cerebral ischemia/reperfusion rats， which may be related with the down-regulation of NOX2 expression by FPR2.
