Quality Value Transmitting of Substance Benchmark of Xiebaisan
10.13422/j.cnki.syfjx.20211346
- VernacularTitle:泻白散的物质基准量值传递分析
- Author:
Yan WANG
1
;
Yan GAO
1
;
Ju-yuan LUO
1
;
Qi QI
1
;
Qing ZHANG
1
;
Rui ZHOU
1
;
Hai-yan HU
1
;
Xiang-long YU
1
;
Shou-ying DU
1
;
Yang LU
1
;
Jie BAI
1
Author Information
1. School of Chinese Materia Medica，Beijing University of Chinese Medicine，Beijing 102488，China
- Publication Type:Research Article
- Keywords:
famous classical formulas;
Xiebaisan;
substance benchmark;
quality value transmitting;
fingerprint;
high performance liquid chromatography （HPLC）;
dry extract yield
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(21):1-9
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Based on fingerprint， index component content and dry extract yield， a quality evaluation method for substance benchmark of Xiebaisan was established to study the key quality attributes， to explore the quantitative transfer relationship between decoction pieces and substance benchmark， and to preliminarily formulate the quality standard of substance benchmark of Xiebaisan. Method:The substance benchmark of Xiebaisan was prepared according to the records of ancient formulas， fingerprints of 15 batches of decoction pieces and substance benchmarks were collected by high performance liquid chromatography （HPLC） and the index components were determined with the mobile phase of acetonitrile-0.05% phosphoric acid solution for gradient elution. The dry extract yield， fingerprint similarity and transfer rate of index components were combined to study the quantity value transmitting. Result:Ten characteristic peaks were identified in fingerprint of the substance benchmark and two characteristic peaks from stir-fried Mori Cortex， four characteristic peaks from baked Lycii Cortex， four characteristic peaks from Glycyrrhizae Radix et Rhizoma Praeparata cum Melle. Mulberroside A， liquiritin and glycyrrhizic acid were used as index components for the determination， the contents of mulberroside A， liquiritin and glycyrrhizic acid in substance benchmark of Xiebaisan were 2.69%-4.26%， 0.09%-0.17% and 0.09%-0.16%， and their transfer rates were （31.37±4.14）%， （36.12±4.03）% and （12.25±0.88）%， respectively. The similarity of fingerprint of substance benchmarks was good， the fingerprint similarities of 14 batches of substance benchmarks and control fingerprint were >0.9. The dry extract yield of substance benchmark of Xiebaisan ranged from 8.09% to 11.29%. Conclusion:The established quality evaluation method of substance benchmark of Xiebaisan is scientific and reasonable， and the transfer process of decoction pieces to substance benchmarks is stable and controllable. The preliminary quality standard of the substance benchmark can provide basis and reference for the development of modern preparations of Xiebaisan in the future.
