Effect and Mechanism of Fuzheng Touxie Prescription on Immune Homeostasis of Drug-resistant Pseudomonas aeruginosa Lung Infection Rats
10.13422/j.cnki.syfjx.20212491
- VernacularTitle:扶正透邪方对耐药铜绿假单胞菌肺部感染大鼠免疫稳态的影响及机制
- Author:
Di ZHANG
1
;
Jun YAN
2
;
Ying QIAN
1
;
Xing-wei YAO
1
;
Yue-feng FU
1
;
Nan GUO
1
;
Bao-ping LI
1
;
Qi YANG
1
;
Qing-quan LIU
3
;
Ling-bo KONG
1
Author Information
1. Dongzhimen Hospital Beijing University of Chinese Medicine，Beijing 100700，China
2. Beijing University of Chinese Medicine Fourth Clinical Medical College，Zaozhuang 277100，China
3. Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University，Beijing 100010，China
- Publication Type:Research Article
- Keywords:
drug-resistant Pseudomonas aeruginosa;
lung infection model rats;
immune homeostasis;
Fuzheng Touxie prescription
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(24):71-77
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of Fuzheng Touxie prescription （FZTX） on the immune homeostasis of drug-resistant Pseudomonas aeruginosa lung infection in rats at different time points. Method:A total of 168 rats were divided into a blank group （n=8），a model group （n=40），a Touxie （TX） group （n=40），an early Fuzheng （FZ） group （n=40）， and a delayed FZ group （n=40）. The blank group was given distilled water by gavage， the model group was given distilled water by gavage after infection，the TX group was given clear heat and penetrate evil drug free decoction granules（3.5 g·kg-1） by gavage after infection， the early FZ group was given Fuzheng Touxie whole formula free decoction granules（10.75 g·kg-1） by gavage after infection， the delayed FZ group was given clear heat and penetrate evil drug free decoction granules by gavage after infection， on the third day plus Fuzheng drug free decoction granules［（3.5+10.75） g·kg-1］ by gavage， the three treatment groups were gavaged twice a day， 2 mL each time .Each drug treatment group was divided into five groups according to five time points （3 h，1 d，3 d，5 d， and 7 d）， with eight rats in each group. The levels of tumor necrosis factor-α（TNF-α），high mobility group protein 1（HMGB1），interleukin-10（IL-10）， and tumor necrosis factor -α-induced protein-8-like2 （TIPE2） were measured by enzyme-linked immunosorbent assay （ELISA）， and HMGB1 protein expression level by Western blot. Result:At 3 h，the TNF-α content in the drug treatment groups was higher than that in the blank group and the model group （P<0.05）. At 3 d，the TNF-α content in the early FZ group and the delayed FZ group was lower than that in the model group （P<0.05） and the TX group （P<0.05）. At 1 d，the HMGB1 content in the TX group and the delayed FZ group was higher than that in the model group （P<0.05）. At 5 d，the HMGB1 content was lower in the delayed FZ group than in the model group （P<0.05）. At 7 d，HMGB1 protein expression in the model group was higher than that in the blank group （P<0.05） and the early FZ group （P<0.05）. At 3 d，the IL-10 content was significantly higher in both the early FZ group and the delayed FZ group than that in the model group （P<0.05）. At 5 d，the IL-10 content was higher in the early FZ group than that in the TX group （P<0.05）. At 7 d，the IL-10 content in the early FZ group and the delayed FZ group was lower than that in the TX group （P<0.05）. At 5 d，the TIPE2 content in the early FZ group was lower than that in the model group （P<0.05）. At 7 d，the TIPE2 content in the TX group and the delayed FZ group was lower than that in the model group （P<0.05）. Conclusion:FZTX or modified prescription can promote the inflammatory response to eliminate pathogenic bacteria in the early stage and suppress the inflammatory response in the late stage to avoid the inflammatory cascade effect and lung tissue damage，indicating that Fuzheng drugs have an important role in maintaining the immune homeostasis of the body after infection.
