Effect of Different Extracts and Monomers of Lepidium meyenii on Proliferation of Mouse Splenic Lymphocyte and Induction of IL-2 and TNF-α
10.13422/j.cnki.syfjx.20212304
- VernacularTitle:玛咖不同提取物和单体对小鼠脾淋巴细胞增殖及诱生IL-2和TNF-α的影响
- Author:
Ming-li LI
1
;
Bao-lin BIAN
1
;
Hong-jie WANG
1
;
Nan SI
1
;
Xiao-lu WEI
1
;
Hai-yu ZHAO
1
;
Yan-yan ZHOU
1
Author Information
1. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700，China
- Publication Type:Research Article
- Keywords:
Lepdium meyenii （Maca）;
splenic lymphocyte;
cell counting kit-8 （CCK-8）;
enzyme-linked immunosorbent assay （ELISA）;
interleukin-2 （IL-2）;
tumor necrosis factor-α （TNF-α）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(23):97-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore effects of different extracts and monomers of Lepidium meyenii （Maca） on the proliferation of mouse splenic lymphocytes and induction of interleukin-2 （IL-2） and tumor necrosis factor-α （TNF-α） by observing their immunomodulatory effects. Method:An octadecylsilyl （ODS） column was used to enrich the methanol extract of L. meyenii in stages to obtain six fractions and three monomers. Different groups of extracts and monomers of L. meyenii at different doses were set up. Cell counting Kit-8 （CCK-8） was used to detect the effect on the proliferation of mitogen-free， concanavalin A （Con A）-induced， and lipopolysaccharides （LPS）-induced mouse splenic lymphocytes. Enzyme-linked immunosorbent assay （ELISA） was used to determine the levels of IL-2 and TNF-α. Result:L. meyenii extracts Fr3 and Fr6， and monomers N-benzyl hexadecanamide and 1，2-dihydro-4-carboxaldehyde-3-benzyl-N-hydroxypyridine slightly promoted the proliferation of Con A-induced T lymphocytes and LPS-induced B lymphocytes （P<0.01） as compared with the conditions in the model group. L. meyenii extracts and monomers significantly induced the secretion of IL-2 and TNF-α by splenic lymphocytes （P<0.01）. Conclusion:L. meyenii extracts and monomers can achieve immunological enhancement by promoting the secretion of IL-2 and TNF-α， and facilitate the proliferation of splenic lymphocytes. The active components are presumedly macamides and pyridine alkaloids， and the specific mechanism still needs to be further explored.
