Analysis on Chemical Constituents in Xiao Chengqitang by UPLC-Q-Orbitrap-MS
10.13422/j.cnki.syfjx.20211450
- VernacularTitle:经典名方小承气汤中化学成分的UPLC-Q-Orbitrap-MS分析
- Author:
Si-ni LUO
1
;
Zhi-cheng PENG
2
;
Qian FAN
2
;
Sheng-kang CAI
2
;
Mei WEI
2
;
Xue-ren CHENG
2
;
Dong-mei SUN
2
Author Information
1. School of the Fifth Clinical Medicine，Guangzhou University of Chinese Medicine，Guangzhou 510405，China
2. Guangdong Efong Pharmaceutical Co. Ltd.，Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule，Foshan 528244，China
- Publication Type:Research Article
- Keywords:
Xiao Chengqitang;
ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry （UPLC-Q-Orbitrap-MS）;
famous classical formulas;
anthraquinones;
anthrones;
flavonoids;
phenylpropanoids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(23):1-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To rapidly identify the chemical constituents in Xiao Chengqitang by ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry （UPLC-Q-Orbitrap-MS）. Method:The method was established by the Waters CORTECS T3 column （2.1 mm×150 mm， 1.6 μm）， mobile phase was methanol （A）-0.1% formic acid aqueous solution （B） for gradient elution （0-5 min， 3%-21%A； 5-20 min， 21%-36%A； 20-32 min， 36%-50%A； 32-42 min， 50%-62%A； 42-50 min， 62%-85%A； 50-60 min， 85%-95%A）， the flow rate was 0.2 mL·min-1， and the column temperature was 30 ℃. UPLC-Q-Orbitrap-MS was operated in positive and negative ion modes， the scanning range was 100-1 200 with mode of Full MS/dd-MS2， and the collision energies were 20， 40 eV. The compounds were identified by comparing with reference substances and combining with literature reports and MS database information. Result:A total of 123 components were identified in Xiao Chengqitang， including 33 flavonoids， 25 anthraquinones and anthrones， 23 phenylpropanoids， 15 tannins， 10 nitrogen-containing components and 17 other components. Among them， 32 components were determined by reference substances. Conclusion:The material basis of Xiao Chengqitang is flavonoids， anthraquinones and anthrones， phenylpropanoids， which is derived from Aurantii Fructus Immaturus， Rhei Radix et Rhizoma and Magnoliae Officinalis Cortex， respectively.
