Effect of Qingkailing on Expression of Toll-like Receptor 4, gp91phox and Zonula Occludens-1 in Cerebrovascular Endothelial Cells Induced by Hypoxia Activating Microglias
10.3969/j.issn.1006-9771.2019.00.013
- VernacularTitle:清开灵对小胶质细胞缺氧活化诱导的脑微血管内皮细胞Toll样受体4、gp91phox和闭锁小带蛋白-1表达的影响
- Author:
Chao TIAN
1
;
Lu-lu MANA
1
;
Meng-chen YUAN
1
;
Li-qin WANG
1
;
Na AN
1
;
Han-lai ZHANG
1
;
Yan-wei XING
2
;
Yi-kun SUN
1
;
Yong-hong GAO
1
Author Information
1. Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
2. Guang’anmen Hospital, Chinese Academy of Chinese Medical Sciences, Beijing 100053, China
- Publication Type:Research Article
- Keywords:
ischemic stroke;
Qingkailing injection;
blood-brain barrier;
microglia;
endothelial cell
- From:
Chinese Journal of Rehabilitation Theory and Practice
2019;25(11):1303-1308
- CountryChina
- Language:Chinese
-
Abstract:
Objetive To investigate the effect of Qingkailing injection on the expression of Toll-like receptor 4 (TLR4), gp91phox and zonula occludens-1 (ZO-1) in cerebrovascular endothelial cells induced by hypoxia activation of microglias. Methods:BV2 microglia cells were divided into six groups. They were cultured in serum-free DMEM, while the Qingkailing groups of low, middle and high dosages were cultured with 0.0625%, 0.125% and 0.25% Qingkailing injection, respectively, and minocycline group with minocycline of 200 nmol/L. The groups other than control group underwent hypoxia for 24 hours and reoxygenation for 24 hours. Then, the medium of microglia was put into the medium of Balb/c endothelial cells for 24 hours. The cell viability of endothelial cells was measured with CCK-8, the concentration of nitric oxide (NO) was detected with colorimetry, and the experission of TLR4, gp91phox and ZO-1 was detected with Western blotting. Results:Compared with the control group, the cell viability and the expression of ZO-1 decreased in the model group (P < 0.01), while the concentration of NO and the expression of TLR4 and gp91phox increased (P < 0.05). Compared with the model group, the cell viability and the expression of ZO-1 increased in the Qingkailing groups and the minocycline group (P < 0.05), while the concentration of NO and the expression of TLR4 and gp91phox decreased (P < 0.05). Conclusion:Qingkailing injection may enhance the survival and function of cerebrovascular endothelial cells by inhibiting the hypoxia activation of microglias, reducing the expression of TLR4 and gp91phox, and increasing the expression of ZO-1.
