Preparation of Rat Spinal Synaptosome and Qualitative and Quantitative Test of Neurotransmitters
10.3969/j.issn.1006-9771.2019.06.010
- VernacularTitle:大鼠脊髓突触体的制备及神经递质的定性和定量分析
- Author:
Zheng LIU
1
;
Jing WANG
2
;
Wei SHEN
1
;
Shu-xuan ZHAO
1
;
Wen-hua YUAN
1
;
Xiao-fei FENG
1
;
Hai-yu ZHOU
1
Author Information
1. Department of Orthopedics, Second Hospital of Lanzhou University, Lanzhou, Gansu 730030, China
2. Department of Special Medicine, The 943th Hospital of the Chinese People's Liberation Army, Wuwei, Gansu 733000, China
- Publication Type:Research Article
- Keywords:
spinal synaptosomes;
neurotransmitters;
ultra performance liquid chromatography;
mass spectrometer;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2019;25(6):670-675
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare rat spinal synaptosomes, and to conduct qualitative and quantitative analysis of various neurotransmitters in rat spinal synaptosomes to explore the physiological activities. Methods:Rat spinal synaptosomes were prepared by discontinuous Percoll density gradient centrifugation and their morphology and structure were observed under transmission electron microscopy. The properties and content of neurotransmitters in rat spinal synaptosomes were determined by ultra performance liquid chromatography-tandem mass spectrometry. Results:Under the experimental conditions, rat spinal synaptosomes were found to contain dopamine, norepinephrine, serotonin, homovanillic acid, 3,4-dihydroxybenzene, 5-hydroxyindoleacetic acid, methoxytyramine, tryptophan, γ-aminobutyric acid, kynurenine, 3-hydroxy kynurenine and 3-hydroxy-2-aminobenzoic acid, and so on. The corresponding neurotransmitter contents were detected, and the corresponding linear relationship and correlation coefficient were obtained. Conclusion:Discontinuous Percoll density gradient centrifugation could prepare rat spinal synaptosomes and detect neurotransmitters and their contents in rat spinal synaptosomes.
