Application of metagenomics in the diagnosis of ophthalmic diseases
10.3980/j.issn.1672-5123.2021.12.13
- VernacularTitle:宏基因组学技术在感染性眼病诊断中的应用
- Author:
Jin-Xia Yu
1
Author Information
1. <sup>1</sup>Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China|<sup>2</sup>Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250002, Shandong Province, China|<sup>3</sup>Emergency Center, Yishui County People's Hospital, Linyi 276400, Shandong Province, China|<sup>4</sup>Institute of Ophthalmology Disease Prevention and Control of Shandong, Jinan 250002, Shandong Province, China
- Publication Type:Journal Article
- Keywords:
metagenomics;
pathogenic microorganism;
keratitis;
dry eye;
endoophthalmitis;
uveitis
- From:
International Eye Science
2021;21(12):2090-2095
- CountryChina
- Language:Chinese
-
Abstract:
In recent years, metagenomics(MGS)is the fastest growing fields in microbiology, and has been broadly applied in the detection of pathogenic microorganisms. Comparing with the traditional poly merase chain reaction(PCR)-based detecting technology which relies on microbial culture, MGS can directly detect the sequences of the total microbial DNA from uncultured samples with the high-throughput sequencing platform. It can help the doctors identify the involved pathogens more quickly and provide better medication guidance. Among the known ophthalmic diseases, a lot of them are caused by the infection of pathogens and have many difficulties in clinical diagnosis and treatment. The development of metagenomics provides us a more effective and reliable way for detecting the pathogens of ophthalmic diseases. This article was aimed to review the development of MGS, applications and in the field of ophthalmology, as well as its current deficiencies and the possible development directions in the future.
- Full text:202112013.pdf
