Analysis of components of proteins from Echinococcus granulosus cyst fluid
10.16250/j.32.1374.2021111
- VernacularTitle:细粒棘球蚴囊液蛋白组分分析
- Author:
Shu-Ying WANG
1
;
Xiao-Di YANG
2
;
Hong-Yu GAO
3
;
Jing-Yu XING
4
;
Qi HU
4
;
Ting-Ting HUANG
4
;
Pei WU
4
;
Yi-Ting ZHAO
4
;
Hao-Wei LIU
4
;
Wei-Yue LIU
4
;
Hui-Na WANG
4
;
Rui ZHOU
5
;
Liang CHU
6
Author Information
1. Department of Pediatrics, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China; Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, China; Co-first authors
2. Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, China; Department of Microbiology and Parasitology, Bengbu Medical College, China; Co-first authors
3. Department of Pediatrics, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China; Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, China
4. Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, China
5. Department of Pediatrics, The First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China
6. Department of General Surgery, The Second Affiliated Hospital of Bengbu Medical College, China
- Publication Type:Journal Article
- Keywords:
Echinococcus granulosus;
Cyst fluid;
Proteomics;
Shotgun;
Immune dysregulation disease
- From:
Chinese Journal of Schistosomiasis Control
2021;33(5):476-482
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the components of proteins from Echinococcus granulosus cyst fluid using the shotgun method, and to identify the active components with potential regulatory effects for immune dysregulation diseases. Methods The E. granulosus cyst fluid was collected aseptically from the hepatic cysts of patients with cystic echinococcosis, and characterized by liquid chromatography (LC) tandem mass spectrometry (MS/MS) following digestion with trypsin. The protein data were searched using the software MaxQuant version 1.6.1.0 and the cellular components, molecular functions, and biological processes of the identified proteins were analyzed using the Gene Ontology (GO) method. Results The E. granulosus cyst fluid separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) had a relative molecular mass of 25 to 70 kDa. LS-MS/MS analysis identified 37 proteins, including 32 known proteins and 5 unknown proteins. At least 4 proteins were preliminarily found to exhibit potential regulatory effects for immune dysregulation diseases, including antigen B, glutathione-S-transferase (GST), thioredoxin peroxidase (TPX) and malate dehydrogenase (MDH). GO enrichment analysis showed that the identified proteins had 149 molecular functions and were involved in 341 biological processes. Conclusions E. granulosus cyst fluid has a variety of protein components, and four known proteins are preliminarily identified to be associated with immune dysregulation diseases.
