Effects of Incremental Load Training on Phosphorylation of AMPK in Skeletal Muscle Satellite Cells of Aged Mice
10.16156/j.1004-7220.2021.04.17
- VernacularTitle:负荷渐增式训练对老年小鼠骨骼肌卫星细胞AMPK磷酸化的影响
- Author:
Yanhua CHEN
1
;
Fan BU
2
;
Bin SHU
1
;
Zhong YANG
3
Author Information
1. Rehabilitation Center of the Fourth Clinical College, Chongqing Medical University
2. Department of Chinese Medicine Rehabilitation, the Affiliated Rehabilitation Hospital of Chongqing Medical University
3. Department of Clinical Hematology, Faculty of Pharmacy and Laboratory Medicine, Army Medical University
- Publication Type:Journal Article
- Keywords:
muscle satellite cells;
incremental load training;
AMP-activated protein kinase (AMPK);
phosphorylation
- From:
Journal of Medical Biomechanics
2021;36(4):E612-E617
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of incremental load training on AMP-activated protein kinase (AMPK) phosphorylation in skeletal muscle satellite cells of aged mice. Methods Experimental mice were divided into 3 groups: young control group (YC group, n=12), old control group (OC group, n=12) and old training group (OT group, n=12). The mice in OT group received incremental load training, and CD45-/CD31-/Sca1-/VCAM (CD106) + cells were isolated by flow cytometry sorting. Desmin, Myod myogenic staining and myogenic differentiation culture were used for identification of muscle satellite cells, and the p-AMPK level of muscle satellite cells was detected by immunohistochemistry combined with Western blotting method. Results The expression levels of AMPK and p-AMPK in skeletal muscle satellite cells in YC group were significantly higher than those in OC group (P<0.05). AMPK expression in OT group and OC group did not change significantly (P>0.05), while p-AMPK expression level in OT group was significant higher than that in OC group (P<0.05). Conclusions Incremental load training can promote AMPK phosphorylation of skeletal muscle satellite cells in aged mice, and improve energy metabolism of skeletal muscle in aged mice.
