Mechanism of sodium salicylate-induced damage to HEI-OC1 cells based on metabonomics
10.11665/j.issn.1000-5048.20210508
- VernacularTitle:基于代谢组学的水杨酸钠对HEI-OC1细胞损伤作用机制
- Author:
Mingzhe HE
1
;
Ying PENG
;
Guangji WANG
;
Jiye A
;
Yiwen ZHENG
;
Jianguo SUN
Author Information
1. 中国药科大学药物代谢动力学重点实验室
- Publication Type:Journal Article
- Keywords:
sodium salicylate;
metabonomics;
HEI-OC1 cell;
biomarkers
- From:
Journal of China Pharmaceutical University
2021;52(5):566-572
- CountryChina
- Language:Chinese
-
Abstract:
The effect of sodium salicylate on the endogenous metabolism of hair cell-like cells (HEI-OC1).of mice was analyzed based on liquid chromatography-quadrupole time of flight mass spectrometry (LC-Q-TOF/MS).Firstly, HEI-OC1 cells were treated with different concentrations of sodium salicylate, and cell survival was examined by the CCK-8 method. Next, sodium salicylate was administered for different duration to observe the changes in cell morphology. Inter-group differential metabolites were screened out, and the associated metabolic pathways were analyzed based on metabonomic technology.Results showed that sodium salicylate could significantly inhibit the survival rate of HEI-OC1 cells, and that, as the concentration increased, the inhibitory effect became stronger. Also, the cell morphology could be elongated after administration and return to normal after withdrawal.Eighteen differential metabolites such as orotic acid, uridine and aspartic acid were screened out after treatment of sodium salicylate, which mainly involving two possible metabolic pathways, namely the metabolism of alanine, aspartic acid and glutamic acid, and that of pyrimidine.In summary, the application of metabolomics technology to evaluate the effect of sodium salicylate on hair cells from the microscopic perspective can provide new ideas for the study of sodium salicylate ototoxicity and development of tinnitus.
