Allomyrina dichotoma larva extract attenuates free fatty acid-induced lipotoxicity in pancreatic beta cells
- Author:
Kyong KIM
1
;
Min-Kyu KWAK
;
Gong-Deuk BAE
;
Eun-Young PARK
;
Dong-Jae BAEK
;
Chul-Young KIM
;
Se-Eun JANG
;
Hee-Sook JUN
;
Yoon Sin OH
Author Information
- Publication Type:Original Research
- From:Nutrition Research and Practice 2021;15(3):294-308
- CountryRepublic of Korea
- Language:English
-
Abstract:
RESULTS:The administration of ADLE to HFD-induced diabetic mice reduced the hyperplasia, 4-hydroxynonenal levels, and the number of apoptotic cells while improving the insulin levels compared to the HFD group. Treatment of INS-1 cells with palmitate reduced insulin secretion, which was attenuated by the ADLE treatment. Furthermore, the ADLE treatment prevented palmitate-induced cell death in INS-1 cells and isolated islets by reducing the apoptotic signaling molecules, including cleaved caspase-3 and PARP, and the Bax/Bcl2 ratio. ADLE also reduced the levels of reactive oxygen species generation, lipid accumulation, and nitrite production in palmitate-treated INS-1 cells while increasing the ATP levels. This effect corresponded to the decreased expression of inducible nitric oxide synthase (iNOS) mRNA and protein.
CONCLUSIONS:ADLE helps prevent lipotoxic beta-cell death in INS-1 cells and HFD-diabetic mice, suggesting that ADLE can be used to prevent or treat beta-cell damage in glucose intolerance during the development of diabetes.
