Protective Effects of KH-204 in the Bladder of Androgen-Deprived Rats.
10.5534/wjmh.2015.33.2.73
- Author:
Woong Jin BAE
1
;
U Syn HA
;
Jin Bong CHOI
;
Kang Sup KIM
;
Su Jin KIM
;
Hyuk Jin CHO
;
Sung Hoo HONG
;
Ji Youl LEE
;
Zhiping WANG
;
Sung Yeoun HWANG
;
Sae Woong KIM
Author Information
1. Catholic Integrative Medicine Research Institute, College of Medicine, The Catholic University of Korea, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Antioxidants;
Hypogonadism;
Phytotherapy;
Testosterone;
Urinary bladder
- MeSH:
Administration, Oral;
Animals;
Antioxidants;
Fibrosis;
Guanosine Monophosphate;
Humans;
Hypogonadism;
Leuprolide;
Male;
Neurons;
Nitric Oxide;
Nitric Oxide Synthase;
Oxidative Stress;
Phytotherapy;
Rats*;
Rho Guanine Nucleotide Exchange Factors;
Superoxide Dismutase;
Testosterone;
Urinary Bladder*;
Water
- From:The World Journal of Men's Health
2015;33(2):73-80
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: We investigated the protective effects of the herbal formulation KH-204 in the bladder of androgen-deprived rats. MATERIALS AND METHODS: Male rats aged eight weeks were randomly divided into four groups, containing eight rats each: sham operation only (normal control group), androgen-deprived only (androgen-deprived control group), and androgen-deprived followed by treatment with 200 mg/kg or 400 mg/kg of KH-204. After 0.5 mg/kg of leuprorelin was subcutaneously injected in the androgen-deprived groups, the oral administration of either distilled water in the two control groups or KH-204 in the treatment group was continued for four weeks. Serum testosterone levels, RhoGEF levels, nitric oxide (NO)-cyclic guanosine monophosphate (cGMP)-related parameters, oxidative stress, and histologic changes were evaluated after treatment. RESULTS: Treatment with the herbal formulation KH-204 (1) increased serum testosterone levels; (2) restored the expression of RhoGEFs, endothelial NO synthase, and neuronal NO synthase; (3) increased the expression of superoxide dismutase; and (4) decreased bladder fibrosis. CONCLUSIONS: Our results suggest that the positive effects of KH-204 on the urinary bladder may be attributed to its antioxidant effects or to an elevation in NO-cGMP activity.
