Quercetin attenuates the reduction of parvalbumin in middle cerebral artery occlusion animal model
10.1186/s42826-021-00086-0
- Author:
Dong-Ju PARK
1
;
Ju-Bin KANG
;
Fawad-Ali SHAH
;
Phil-Ok KOH
Author Information
1. Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, 501 Jinju-daero, 52828, Jinju, South Korea
- Publication Type:R E S E A R C H
- From:Laboratory Animal Research
2021;37(1):74-81
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Calcium is a critical factor involved in modulation of essential cellular functions. Parvalbumin is a calcium buffering protein that regulates intracellular calcium concentrations. It prevents rises in calcium concentrations and inhibits apoptotic processes during ischemic injury. Quercetin exerts potent antioxidant and anti-apoptotic effects during brain ischemia. We investigated whether quercetin can regulate parvalbumin expression in cerebral ischemia and glutamate toxicity-induced neuronal cell death. Adult male rats were treated with vehicle or quercetin (10 mg/kg) 30 min prior to middle cerebral artery occlusion (MCAO) and cerebral cortical tissues were collected 24 h after MCAO. We used various techniques including Western blot, reverse transcriptionPCR, and immunohistochemical staining to elucidate the changes of parvalbumin expression.
Results:Quercetin ameliorated MCAO-induced neurological deficits and behavioral changes. Moreover, quercetin prevented MCAO-induced a decrease in parvalbumin expression.
Conclusions:These findings suggest that quercetin exerts a neuroprotective effect through regulation of parvalbumin expression.
