Analysis of cell survival genes in human gingival fibroblasts after sequential release of trichloroacetic acid and epidermal growth factor using the nano-controlled release system
10.14368/jdras.2020.36.3.145
- Author:
Joon Youn CHO
1
;
Richard LEESUNGBOK
;
Suk Won LEE
Author Information
1. Department of Biomaterials & Prosthodontics, Kyung Hee University Hospital at Gangdong, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea
- Publication Type:Original Article
- From:Journal of Dental Rehabilitation and Applied Science
2020;36(3):145-157
- CountryRepublic of Korea
- Language:English
-
Abstract:
Purpose:This study was to determine the possible effects of trichloroacetic acid (TCA) and epidermal growth factor (EGF) through cell survival genes of the PI3K-AKT signaling pathway when applying an hydrophobically modified glycol chitosan (HGC)-based nanocontrolled release system to human gingival fibroblasts in oral soft tissue regeneration.
Materials and Methods:An HGC-based nano-controlled release system was produced, followed by the loading of TCA and EGF. The group was divided into control (CON), TCA-loaded nano-controlled release system (EXP1), and the TCA- and EGF- individually loaded nano-controlled release system (EXP2).A total for 29 genes related to the PI3K-AKT signaling pathway were analyzed after 48h of culture in human gingival fibroblasts.Real-time PCR, 1- way ANOVA and multiple regression analysis were performed.
Results:Cell survival genes were significantly upregulated in EXP1 and EXP2. From multiple regression analysis, ITGB1 was determined to be the most influential factor for AKT1 expression.
Conclusion:The application of TCA and EGF through the HGC-based nano-controlled release system can up-regulate the cell survival pathway.
