Sulfite as the substrate of C-sulfonate metabolism of α, β-unsaturated carbonyl containing andrographolide: analysis of sulfite in rats' intestinal tract and the reaction kinetics of andrographolide with sulfite.
10.1016/S1875-5364(21)60094-8
- Author:
Zhi-Peng HUO
1
;
Xin-Chi FENG
2
;
Yu WANG
3
;
Yu-Ting TIAN
4
;
Feng QIU
5
,
6
Author Information
1. School of Chinese Materia Medica, and State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; TCM Research Center, Tasly Academy, Tasly Holding Group Co., Ltd., Tianjin 300402, China; State Key Laboratory of Critical Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Co., Ltd., Tianjin 300402, China. Electronic address: huozhipeng2012@foxmail.com.
2. School of Chinese Materia Medica, and State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
3. TCM Research Center, Tasly Academy, Tasly Holding Group Co., Ltd., Tianjin 300402, China; State Key Laboratory of Critical Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Co., Ltd., Tianjin 300402, China; Institute of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
4. TCM Research Center, Tasly Academy, Tasly Holding Group Co., Ltd., Tianjin 300402, China; State Key Laboratory of Critical Technology in Innovative Chinese Medicine, Tasly Pharmaceutical Co., Ltd., Tianjin 300402, China; Institute of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
5. School of Chinese Materia Medica, and State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. Electronic address: fengqiu20070118@
6. com.
- Publication Type:Journal Article
- Keywords:
Andrographolide;
C-Sulfonate metabolites;
Non-enzymatic;
Reaction kinetics;
Sulfite;
α, β-Unsaturated carbonyl
- From:
Chinese Journal of Natural Medicines (English Ed.)
2021;19(9):706-712
- CountryChina
- Language:English
-
Abstract:
One-sixth of the currently known natural products contain α, β-unsaturated carbonyl groups. Our previous studies reported a rare C-sulfonate metabolic pathway. Sulfonate groups were linked to the β-carbon of α, β-unsaturated carbonyl-based natural compounds through this pathway. However, the mechanism of this type of metabolism is still not fully understood, especially whether it is formed through enzyme-mediated biotransformation or direct sulfite addition. In this work, the enzyme-mediated and non-enzymatic pathways were studied. First, the sulfite content in rat intestine was determined by LC-MS/MS. The results showed that the amount of sulfite in rat intestinal contents was from 41.5 to 383 μg·g
