Pharmacokinetic study of Polygonum orientale extract in H9c2 cells by UPLC-MS/MS.
10.19540/j.cnki.cjcmm.20210618.202
- Author:
Yuan LU
1
;
Na LI
2
;
Ting LYU
2
;
Yong-Lin WANG
1
;
Jie PAN
3
;
Jia SUN
1
;
Yong-Jun LI
3
;
Chun-Hua LIU
3
Author Information
1. Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University Guiyang 550004, China.
2. Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University Guiyang 550004, China School of Pharmaceutical Sciences, Guizhou Medical University Guiyang 550004, China.
3. Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University Guiyang 550004, China.
- Publication Type:Journal Article
- Keywords:
H9c2 cell;
Polygonum orientale;
UPLC-MS/MS;
cellular pharmacokinetics;
mitochondria;
nuclei
- MeSH:
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Drugs, Chinese Herbal;
Polygonum;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(18):4833-4840
- CountryChina
- Language:Chinese
-
Abstract:
A detection method of ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was established to detect concentrations of isoorientin, orientin, quercetin, vitexin and kaempferol-3-O-β-D-glucoside in H9 c2 cells and applied to the pharmacokinetic study of Polygonum orientale extract in the cells. H9 c2 cells were treated with 100 μg·mL~(-1) P. orientale extract and then they and the corresponding nuclei, mitochondria and Golgi bodies were collected at the set time. After protein precipitation, UPLC-MS/MS was used to determine concentrations of isoorientin, orientin, quercetin, vitexin and kaempferol-3-O-β-D-glucoside in the whole cells and subcellular structures. Also, related pharmacokinetic parameters were calculated. The results showed that the peak time was 8 h for all these components. Orientin, vitexin, quercetin and isoorientin have high affinities to nuclei and mitochondria, while the affinity of kaempferol-3-O-β-D-glucoside is higher with mitochondria compared to nuclei. It is suggested that these chemical components of P. orientale may mainly act on nuclei or mitochondria to exert pharmacological effects of protecting cardiomyocytes.
