Spectrum-effect relationship of the blood-activating efficacy of Notoginseng Radix et Rhizoma.
10.19540/j.cnki.cjcmm.20210127.301
- Author:
Yong-Sheng DING
1
;
Qiu-Yu LI
2
;
Li-Hua BIAN
2
;
Xiao-Mei WANG
2
;
Zi-Wei YAO
2
;
Cheng-Bo-Wen ZHAO
2
;
Jing LUO
2
;
Jin-Li SHI
2
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China China Resources Double-crane Pharmaceutical Co., Ltd. Beijing 100102, China.
2. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
- Publication Type:Journal Article
- Keywords:
Notoginseng Radix et Rhizoma;
capillary coagulation experiment;
fingerprint;
middle cerebral artery occlusion(MCAO)-induced ce-rebral ischemia-reperfusion injury;
promoting blood circulation;
spectrum-effect correlation
- MeSH:
Animals;
Blood Coagulation;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Quality Control;
Rats;
Rhizome;
Saponins
- From:
China Journal of Chinese Materia Medica
2021;46(18):4736-4743
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to establish the high-performance liquid chromatography(HPLC) fingerprints of different batches of Notoginseng Radix et Rhizoma, determine their pharmacodynamic indexes of promoting blood circulation, and explore the spectrum-effect relationship between the chemical components of Notoginseng Radix et Rhizoma and the efficacy of promoting blood circulation. Firstly, the HPLC fingerprints of different batches of Notoginseng Radix et Rhizoma were established. Then, the pharmacodynamic indexes were determined after the capillary coagulation experiment and the cerebral ischemia-reperfusion in rats, including capillary coagulation time, percentage of cerebral ischemic area, cerebral water loss rate, and brain-body index. Afterward, the partial least-squares method was used to explore the spectrum-effect relationship between the chemical components of Notoginseng Radix et Rhizoma and the pharmacodynamic indexes. The results showed that this study successfully established the HPLC fingerprints of different batches of Notoginseng Radix et Rhizoma, found 23 common peaks, and identified 12 of them, all of which were saponins. The method was proved stable and reliable. Both the capillary coagulation experiment and the middle cerebral artery occlusion(MCAO)-induced cerebral ischemia-reperfusion experiment on rats revealed that there were obvious differences in the pharmacodynamic indexes of different batches of Notoginseng Radix et Rhizoma. The relationships between 23 common components of Notoginseng Radix et Rhizoma in different batches and the pharmacodynamic indexes were discussed by means of spectrum-effect correlation analysis, of which 17 components had positive effects while 6 components had negative effects on the pharmacodynamic indexes. This study provides a certain reference basis for the clinical rational use and quality control of Notoginseng Radix et Rhizoma.
