Chemical constituents of Physalis minima.
10.19540/j.cnki.cjcmm.20210525.202
- Author:
Li-Sha CHAI
1
;
Guo-Sheng LIU
1
;
Yu-Xun ZHU
1
;
Shang-Yi WANG
1
;
Cheng-Shuo YANG
1
;
Yong LI
1
Author Information
1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100050, China.
- Publication Type:Journal Article
- Keywords:
NF-κB;
Physalis minima;
anti-inflammatory
- MeSH:
Anti-Inflammatory Agents;
Chromatography, High Pressure Liquid;
NF-kappa B;
Physalis;
Spectrometry, Mass, Electrospray Ionization
- From:
China Journal of Chinese Materia Medica
2021;46(15):3865-3872
- CountryChina
- Language:Chinese
-
Abstract:
Fifteen compounds(1-15) were isolated from the 95% EtOH extract of the whole herb of Physalis minima by various chromatography techniques including silica gel, Sephadex LH-20, middle chromatogram isolated gel(MCI), octadecyl silica(ODS), and semi-preparative high performance liquid chromatography(HPLC). Their structures were elucidated by infrared spectroscopy(IR), ultraviolet spectroscopy(UV), high-resolution electrospray ionization mass spectrometry(HR-ESI-MS), nuclear magnetic re-sonance(NMR), and circular dichroism(CD) as(5S)-5,11-dihydroxy-3-methyl-5-pentylfuran-2(5H)-one(1), withaphysalin R(2), withaphysalin Q(3), withaphysanolide A(4), phaseic acid(5), grasshopper ketone(6), 3S,5R-dihydroxy-6S,7-megastigmadien-9-one(7), vanillic acid(8), 2-trans,4-trans-abscisic acid(9), capillasterolide(10), 5,3'-dihydroxy-3,7,4'-trimethoxyflavone(11),(-)-loliolide(12), 4-hydroxyacetophenone(13), acetosyringone(14), and aurantiamide acetate(15). Compound 1 was a new butenolide, and compounds 5-7 and 10-12 were isolated from the Physalis for the first time. Compounds 4, 13, and 15 were isolated for the first time from P. minima. Moreover, their anti-inflammatory activity was evaluated in vitro. Compound 12 was found to possess an inhibitory effect on the transcription of an NF-κB-dependent reporter gene in LPS-induced 293 T/NF-κB-luc cells at 10 μmol·L~(-1), showing an inhibitory rate of 62.31%±4.8%.
