Quality standard of Perillae Folium based on multicomponent determination with HPLC method.
10.19540/j.cnki.cjcmm.20210507.301
- Author:
Yu YAN
1
;
Hong YI
1
;
Dong ZHANG
1
;
Chun LI
1
;
Liang-Mian CHEN
1
;
Jing-Yuan ZHAO
1
;
Hui-Min GAO
1
;
Li-Hua YAN
1
;
Xiao-Qian LIU
1
;
Zhi-Min WANG
1
Author Information
1. National Engineering Laboratory of Quality Control Technology of Chinese Materia Medica, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Perillae Folium;
caffeic acid;
perillaketone;
perillaldehyde;
rosmarinic acid;
scutellarin
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Plant Extracts;
Plant Leaves
- From:
China Journal of Chinese Materia Medica
2021;46(16):4051-4060
- CountryChina
- Language:Chinese
-
Abstract:
This research established the HPLC methods for the determination of perillaketone, perillaldehyde, caffeic acid, scutellarin, and rosmarinic acid in 33 batches of Perillae Folium. Kromasil C_(18)(4.6 × 250 mm, 5 μm) chromatographic column was used, and the mobile phase for determination of the perillaketone and perillaldehyde was methanol-water(55∶45) solution, at a flow rate of 1.0 mL·min~(-1), with the column temperature at 30 ℃. The mobile phase for the determination of caffeic acid, scutellarin and rosmarinic acid was methanol(A)-0.2% phosphoric acid aqueous solution(B) with gradient elution(0-20 min, 25%-30% A; 20-60 min, 30%-43% A). The flow rate was 1.0 mL·min~(-1) and the column temperature was set at 30 ℃. The results showed that the established method can achieve good separation of the five components in samples, with a good linear relationship and high accuracy, indicating that the methods can be used for the determination of Perillae Folium. The results showed that all samples contained five components. And the content of rosmarinic acid(0.04%-1.57%) > scutellarin(0.03%-0.77%) > perillaldehyde(0.02%-0.66%) > perillaketone(0.03%-0.30%) > caffeic acid(0.006%-0.07%). Thirty-three Batches of Perillae Folium can be grouped into 5 categories. There are certain content rules and region specificities under different clusters. Perillaketone, perillaldehyde, and rosmarinic acid can be used as the main markers to evaluate the quality of Perillae Folium.
