Effect of Danhong Injection on platelet metabolites based on metabonomics technology.
10.19540/j.cnki.cjcmm.20210317.202
- Author:
Tian-Yu LOU
1
;
Bei-Bei MA
1
;
Rui-Ji LI
1
;
Jin-Hui LIU
1
;
Shang-Yue YU
1
;
Ting-Ting WANG
1
;
Zhao-Zhou LIN
2
;
Zhi-Bin WANG
2
Author Information
1. School of Chinese Materia Medica,Beijing University of Chinese Medicine Beijing 100029,China.
2. Beijing Institution of Tongrentang Beijing 100079,China.
- Publication Type:Journal Article
- Keywords:
Danhong Injection;
differential metabolites;
metabolic pathway;
metabonomics;
platelet
- MeSH:
Animals;
Biomarkers;
Blood Platelets;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/pharmacology*;
Metabolomics;
Rabbits;
Technology
- From:
China Journal of Chinese Materia Medica
2021;46(13):3422-3428
- CountryChina
- Language:Chinese
-
Abstract:
The effect of Danhong Injection on the endogenous metabolites of rabbit platelets was analyzed by the liquid chromatography-mass spectrometry( LC-MS) based metabonomic approach. Anti-platelet aggregation was detected after Danhong Injection treatment and the changes of platelet metabolites were analyzed by metabonomics. Principal component analysis( PCA) and partial least squares discriminant analysis( PLS-DA) were performed to investigate the effect of Danhong Injection on endogenous metabolites of platelets,characterize the biomarkers,and explore the relevant pathways and the underlying mechanism. As demonstrated by the pharmacodynamic results,Danhong Injection of different doses and concentrations antagonized platelet aggregation in a dose-and concentration-dependent manner. In contrast to the control group,25 differential metabolites such as nicotinic acid,nicotinic acid riboside,and hypoxanthine were screened out after platelets were treated by Danhong Injection. These metabolites,serving as important biomarkers,were mainly enriched in the nicotinic acid-niacinamide metabolic pathway and purine metabolic pathway. This study explored the therapeutic mechanism of Danhong Injection from a microscopic perspective by metabonomics,which is expected to provide a new idea for the investigation of platelet-related mechanisms.
