Application of CRISPR in evolution analysis, detecting and typing, virulence and antibiotic resistance regulation in food-borne pathogens.

Zhiye BAI; Wen WANG; Xiaofeng JI; Yingping XIAO; Shiqin ZHANG; Zichen WANG; Hongmei LI; Qingli DONG

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Application of CRISPR in evolution analysis, detecting and typing, virulence and antibiotic resistance regulation in food-borne pathogens.

Author: Zhiye BAI ¹ ; Wen WANG ² ; Xiaofeng JI ² ; Yingping XIAO ² ; Shiqin ZHANG ¹ ; Zichen WANG ¹ ; Hongmei LI ¹ ; Qingli DONG ¹
Author Information

1. School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, MOA Laboratory of Quality & Safety Risk Assessment for Agro-products (Hangzhou), Institute of Quality and Standard of Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China.
Publication Type:Review
Keywords: CRISPR; detection and typing; evolutionary analysis; food-borne pathogens; virulence and antibiotic resistance
MeSH: Bacteria/genetics*; Bacteriophages/genetics*; CRISPR-Cas Systems/genetics*; Clustered Regularly Interspaced Short Palindromic Repeats/genetics*; Drug Resistance, Microbial/genetics*; Virulence/genetics*
From: Chinese Journal of Biotechnology 2021;37(7):2414-2424
CountryChina
Language:Chinese
Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR) and its associated protein gene system can limit the horizontal gene transfer, thereby effectively preventing the invasion of foreign gene elements such as bacteriophages. CRISPR arrays of different bacteria are diverse. Based on the differences in the CRISPR system, this review summarizes the application of CRISPR in food-borne pathogen evolution analysis, detection and typing, virulence and antibiotic resistance in recent years. We also address bacterial detection typing method developed based on the characteristics of CRISPR arrays and the association of CRISPR with virulence and drug resistance of food-borne pathogens. The shortcomings of CRISPR in evolution, detection and typing, virulence and resistance applications are analyzed. In addition, we suggest standardizing CRISPR typing methods, improving and expanding the CRISPR database of pathogenic bacteria, and further exploring the co-evolution relationship between phages and bacteria, to provide references for further exploration of CRISPR functions.