Establishment of high-resolution bioassay profiling platform to screen α-glucosidase inhibitors from Malus hupehensis
10.16438/j.0513-4870.2021-0886
- VernacularTitle:基于近线高分辨活性轮廓分析的湖北海棠中α-葡萄糖苷酶抑制剂筛选研究
- Author:
Ling-ling XI
1
;
Jing-yi JIAN
1
;
Ding-sheng ZHA
2
;
Xiang-long ZHAO
3
;
Jin-cai WANG
1
;
Juan LI
4
;
Zheng-jin JIANG
1
;
Ting-ting ZHANG
1
Author Information
1. Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China
2. The First Affiliated Hospital of Jinan University, Guangzhou 510630, China
3. Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China; SCIEX (China) Co., Ltd., Guangzhou 510623, China
4. College of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China
- Publication Type:Research Article
- Keywords:
high-resolution bioassay profiling platform;
italic>Malus hupehensis;
type 2 diabetes;
italic>α-glucosidase inhibitor;
active screening
- From:
Acta Pharmaceutica Sinica
2021;56(9):2419-2425
- CountryChina
- Language:Chinese
-
Abstract:
italic>α-Glucosidase inhibitors play an important role in the treatment of diabetes. This study established a high-resolution bioassay profiling platform for rapidly screening α-glucosidase inhibitors in natural product extracts. Five α-glucosidase inhibitors were identified from Malus hupehensis, namely, 3-hydroxyphloridzin, quercetin-3-O-β-D-glucopyranoside, phloridzin, avicularin and quercitrin. The establishment and successful application of this platform provides a powerful tool for the efficient discovery of anti-diabetic active ingredients in complex systems.
