Mechanism investigation on the enhanced anti-hepatic fibrosis effects in rats of Fructus Corni after wine-processing based on plasma metabonomics
10.16438/j.0513-4870.2021-0652
- VernacularTitle:基于血浆代谢组学的山茱萸酒制后抗大鼠肝纤维化作用增强机制研究
- Author:
Min-jie NIU
1
;
Meng-qing WANG
1
;
Hui YU
1
;
Xin LIU
1
;
Hao CAI
1
;
Gang CAO
2
;
Yu DUAN
1
;
Ke PEI
3
;
Zhuan ZHANG
4
Author Information
1. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China; Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China
2. School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China
3. School of Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine, Jinzhong 030619, China
4. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
- Publication Type:Research Article
- Keywords:
crude and wine-processed Fructus Corni;
hepatic fibrosis;
plasma metabonomics;
mechanism of action
- From:
Acta Pharmaceutica Sinica
2021;56(9):2410-2418
- CountryChina
- Language:Chinese
-
Abstract:
Metabonomics technology was employed to investigate and identify the mechanisms and metabolic pathways of the crude and wine-processed Fructus Corni extracts on anti-hepatic fibrosis effects in rats, and to compare and analyze the potential mechanism of enhanced interference of the wine-processed Fructus Corni on hepatic fibrosis effects in rats. The rats were randomly divided into the blank control group, the model group, the colchicine group, the crude Fructus Corni groups with low, medium, and high-doses, and the wine-processed Fructus Corni groups with low, medium, and high-doses, and there were six rats in each group. The hepatic fibrosis model was established by subcutaneous injection of 40% carbon tetrachloride, and the intragastric administration was performed at the third week of modeling. The blood and liver samples of rats were taken and carried out for pharmacodynamic index detection and UHPLC-Q-TOF-MS/MS analysis after intragastric administration for six weeks. The results of pharmacodynamic investigation showed that both the crude and wine-processed Fructus Corni had the effects of anti-hepatic fibrosis in rats. Metabonomics analysis indicated that, compared to the blank control group, the twenty-four potential biomarkers related to hepatic fibrosis were screened and identified in the model group, which mainly involved in primary bile acid metabolism, glycerol phospholipid metabolism, pentose and glucuronide metabolism, retinol metabolism, and arachidonic acid metabolism. The crude and wine-processed Fructus Corni extracts had different degrees of callback effects on the ten of the above potential biomarkers, and the effect of wine-processed Fructus Corni was better than that of crude one. The present study clarifies the mechanism of enhanced efficiency of wine-processed Fructus Corni from the perspective of plasma metabolism, and provides the theoretical foundation for further development and clinical application of Fructus Corni.
