Comparative study on the protective effect of Salvia miltiorrhiza and Anemarrhena asphodeloides on AD cell model using UHPLC-QTOF/MS based cell metabolomics
10.16438/j.0513-4870.2021-0646
- VernacularTitle:基于UHPLC-QTOF/MS的细胞代谢组学对丹参和知母防治阿尔茨海默病的药效比较研究
- Author:
Hui WANG
1
;
Ying CAI
2
;
Min LIU
3
;
Zhan-ying HONG
2
;
Yi-feng CHAI
1
Author Information
1. School of Pharmacy, Naval Medical University, Shanghai 200433, China
2. School of Pharmacy, Naval Medical University, Shanghai 200433, China; School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
3. Department of Pharmacy, Changhai Hospital, Naval Military Medical University, Shanghai 200433, China
- Publication Type:Research Article
- Keywords:
UHPLC-QTOF/MS;
cell metabolomics;
Alzheimer's disease;
italic>Salvia miltiorrhiza;
italic>Anemarrhena asphodeloides;
comparative efficacy
- From:
Acta Pharmaceutica Sinica
2021;56(9):2394-2402
- CountryChina
- Language:Chinese
-
Abstract:
The paper aims to compare the protective effect of Salvia miltiorrhiza and Anemarrhena asphodeloides on AD cell model and investigate its protective mechanism by cell metabolomics platform. AD cell model was established by the abnormal phosphorylation of Tau protein in SH-SY5Y cells induced by okadaic acid. The protective effect of the extract of Salvia miltiorrhiza and Anemarrhena asphodeloides on the model was evaluated by cell proliferation-toxicity experiment. The metabolomics platform was used to study the efficacy of Salvia miltiorrhiza and Anemarrhena asphodeloides comprehensively, explore the potential biomarkers related to AD and the effect of drugs on the potential biomarkers. Salvia miltiorrhiza extract had a certain protective effect on the AD model (P < 0.05), while the Anemarrhena asphodeloides extract had no significant protective effect (P > 0.05). 45 significant differential metabolites and the related 12 metabolic pathways were identified using UHPLC-QTOF/MS platform, which were related to the AD cell model. After administration of Salvia miltiorrhiza extract, 30 different metabolites appeared callback, while after intervention of Anemarrhena asphodeloides extract, 7 metabolites appeared callback. The results showed that the extracts of Salvia miltiorrhiza and Anemarrhena asphodeloides had certain protective effects on the AD cell model with Tau protein abnormal phosphorylation, but Salvia miltiorrhiza had more extensive targets and could significantly improve the cell viability. The mechanism may be related to the regulation of the metabolic pathways of AD cell model induced by okadaic acid.
