Analysis of monosaccharide composition and monosaccharide ratio in propylene glycol alginate sodium sulphate drugs by high performance anion exchange chromatograghy -pulsed amperometric detection
10.16438/j.0513-4870.2021-0569
- VernacularTitle:高效阴离子交换-脉冲安培法分析藻酸双酯钠的单糖组成和比例
- Author:
Yue WANG
1
;
Xin-tong CHEN
1
;
Zhen-hua LI
2
;
Yu-juan SONG
1
;
Hui-hong FAN
1
Author Information
1. National Institute for Food and Drug Control, Beijing 102629, China; NMPA Key Laboratory for Quality Research and Evaluation of Chemical Drugs, Beijing 102629, China
2. National Institute for Food and Drug Control, Beijing 102629, China
- Publication Type:Research Article
- Keywords:
anion exchange chromatograghy;
pulsed amperometric detection;
monosaccharide composition;
propylene glycol alginate sodium sulphate;
mannuronic acid;
guluronic acid
- From:
Acta Pharmaceutica Sinica
2021;56(9):2346-2351
- CountryChina
- Language:Chinese
-
Abstract:
The paper aims to establish the method to determine the monosaccharide composition and monosaccharide ratio in propylene glycol alginate sodium sulphate (PSS). Samples were hydrolyzed with trifluoroacetic acid, neutralized with sodium hydroxide solution after the reaction conditions for sample pretreatment were optimized via orthogonal analysis. A high performance anion exchange chromatograghy (HPAEC) coupled with pulsed amperometric detector (PAD) was performed on a CarboPac®PA20, using 200 mmol·L-1 sodium hydroxide solution and 1 mol·L-1 sodium acetate solution as mobile phase. The established HPAEC-PAD method was validated by testing the linear relationship, precision and accuracy, and showed exclusive, sensitive, rapid and wide use. The monosaccharide composition of PSS from different manufacture can be accurately determined with great significance for the structural identification of PSS.
