Effects of Virus Inactivation Treatment of Plasma Specimen on Plasma Concentration Determination of Vorico- nazole，Linezolid，Vancomycin and Teicoplanin
- VernacularTitle:血浆标本病毒灭活处理对伏立康唑、利奈唑胺、万古霉素和替考拉宁血药浓度检测的影响
- Author:
Honglian LI
1
;
Qiong ZHOU
1
;
Jun ZHANG
1
;
Hua HUANG
1
;
Jingjing WANG
1
;
Qin YAO
1
Author Information
1. Dept. of Clinical Pharmacy，the First Affiliated Hospital of Kunming Medical University，Kunming 650000，China
- Publication Type:Journal Article
- Keywords:
Voriconazole;
Linezolid;
Vancomycin;
Teicoplanin;
Plasma concentration;
Plasma sample;
Virus inactivation
- From:
China Pharmacy
2021;32(19):2394-2399
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the effects of virus in activation treatment of plasma specimen on plasma concentration determination of voriconzole ，linezolid，vancomycin and teicoplanin. METHODS ：The remaining plasma of 36 inpatients in our hospital after routine blood concentration examination of voriconazole ，linezolid，vancomycin and teicoplanin were collected as specimen（9 drug-contained plasma specimens for each drug ），and merged into three different concentration levels （low，medium， high）of mixed samples according the results of routine blood test. Then the mixed samples with different concentration levels were divided into inactivated group and non-inactivated group ，with 3 samples in each group. The inactivated plasma samples were heated at 56 ℃ for 30 min in metal bath with constant temperature. Non-inactivated group were not treated. After pretreating plasma sample of 2 groups，2-dimensional liquid chromatography was used to detect plasma concentration of the four drugs ；the difference of detection result between inactivated group and non-inactivated group were analyzed. RESULTS ：Plasma samples containing voriconazole，linezolid，vancomycin and teicoplanin were still stable after heating at 56 ℃ for 30 min in metal bath with constant temperature. Compared with non-inactivated group ，relative error of plasma concentration detection result of above 4 drugs were all lower than 15％ in low ，medium，high concentration mixed samples of inactivated group. CONCLUSIONS ：Plasma samples can be inactivated by heating at 56 ℃ for 30 min in metal bath with constant temperature ，when the plasma concentration of voriconazole，linezolid，vancomycin and teicoplanin are determined by 2-dimensional liquid chromatography.
