Study on emergency metabolic changes of Anopheles sinensis larvae following exposure to deltamethrin
10.16250/j.32.1374.2020329
- VernacularTitle:中华按蚊幼虫溴氰菊酯暴露后应急代谢变化研究
- Author:
Ya-Shu LI
1
;
Jian-Xia TANG
1
;
Ju-Lin LI
1
;
Cheng LIANG
1
;
Mei-Hua ZHANG
1
;
Jing-Yao WU
1
;
Guan-Xi WANG
1
;
Guo-Ding ZHU
1
;
Jun CAO
1
Author Information
1. Jiangsu Institute of Parasitic Diseases, WHO Collaborating Centre for Research and Training on Malaria Elimination, Key Laboratory of National Health Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Wuxi 214064, China
- Publication Type:Journal Article
- Keywords:
Anopheles sinensis;
Larvae;
Deltamethrin;
Metabolism
- From:
Chinese Journal of Schistosomiasis Control
2021;33(4):387-395
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the changes of small molecular metabolites in the larvae of a deltamethrin-sensitive strain of Anopheles sinensis following exposure to deltamethrin, so as to provide the scientific basis for investigating the metabolic pathway and screening metabolic markers of deltamethrin in An. sinensis. Methods The 50% and 75% lethal concentrations (LC50 and LC75) of deltamethrin against the larvae of a deltamethrin-sensitive strain of An. sinensis were calculated in laboratory. The type and content of An. sinensis larvae metabolites were detected using high performance liquid chromatography and mass spectrometry (LC-MS/MS) following exposure to deltamethrin at LC50 and LC75 for 30 min and 24 h, and the changes of metabolites were analyzed. Results The LC50 and LC75 values of deltamethrin were 4.36 × 10-3 µg/mL and 1.12 × 10-2 µg/mL against thelarvae of a deltamethrin-sensitive strain of An. sinensis. Following exposure of the larvae of a deltamethrin-sensitive strain of An. sinensis to deltamethrin at LC50 and LC75 for 30 min, the differential metabolites mainly included organic oxygen compounds, carboxylic acid and its derivatives, fatty acyl and pyrimidine nucleotides, with reduced glucose levels. Following exposure for 24 h, the differential metabolites mainly included organic oxygen compounds, carboxylic acid and its derivatives, aliphatic acyl and purine nucleotides, with increased glucose level detected. Conclusion Carbohydrate, carboxylic acid and its derivatives, fatty acyls, amino acids and their derivatives may play important roles in deltamethrin metabolism in the larvae of a deltamethrin-sensitive strain of An. sinensis.
