Effects of Water Extract from Carpesium cernuum on Proliferation ，Metastasis and Invasion of Prostate Cancer PC3 Cells
- VernacularTitle:烟管头草水提物对前列腺癌PC3细胞增殖、迁移与侵袭的影响
- Author:
Bo LIU
1
;
Jia YU
2
;
Kun WANG
3
;
Chen YAN
4
;
Lirong HUANG
5
;
Heng LUO
6
Author Information
1. State Key Laboratory of Functions and Applications of Medicinal Plants，Guizhou Medical University，Guiyang 550014，China;College of Pharmacy，Guizhou Medical University，Guiyang 550025，China
2. State Key Laboratory of Functions and Applications of Medicinal Plants，Guizhou Medical University，Guiyang 550014，China;Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences，Guiyang 550014，China
3. Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences，Guiyang 550014，China
4. Dept. of Pharmacy，Anshun Municipal People’s Hospital，Guizhou Anshun 561000，China
5. College of Food and Pharmaceutical Engineering，Guizhou In stitute of Technology，Guiyang 550003，China
6. State Key Laboratory of Functions and Applications of Medicinal Plants，Guizhou Medical University，Guiyang 550014，China
- Publication Type:Journal Article
- Keywords:
Carpesium cernuum;
Prostate cancer PC 3 cells;
β-catenin;
Proliferation;
Metastasis;
Invasion
- From:
China Pharmacy
2021;32(15):1830-1836
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To study the effe cts of the water extract from Carpesium cernuum （AECC）on the proliferation ， metastasis and invasion of prostate cancer PC 3 cells. METHODS ：Cells were divided into control group and different concentration groups of AECC （5，10，20，40，80 μg/L），and then treated with relevant medicine or medium for different time （24，48，72 h）. The survival rates of cells were detected. Cells were divided into control group ，and AECC low ，medium and high concentration groups（20，40，80 μg/L）. After cultured for 24 h，Hoechst 33258 staining and flow cytometry were used to detect the apoptosis of cells. The number of cell metastasis and invasion were detected by Transwell assay. RT-qPCR and Western blot assay were applied to detect the mRNA and protein expression of β-catenin signaling pathway related migration and apoptosis proteins （β-catenin， MMP-7，c-Myc，caspase-3，Bcl-2 and Bax ）in AECC low and medium concentration groups. RESULTS ：With the increase of the concentration and culture time ，the survival rates of cells in AECC different concentration groups were significantly lower than control group （P＜0.05 or P＜0.01），and showed a decreasing trend. Compared with control group ，the early apoptosis rate （except the medium concentration group ）and the number of cell metastasis and invasion in AECC groups ，the mRNA and protein expression of MMP- 7，c-Myc（except for the low concentration group ）and Bcl- 2（except for mRNA of the low concentration group）in AECC low and medium concentration groups were decreased significantly （P＜0.05 or P＜0.01）. Late apoptosis rate of AECC groups ，the mRNA and protein expression of β-catenin，caspases-3（except for the low concentration group ），Bax（except for mRNA of the low concentration group ）in AECC low and medium concentration groups were increased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS ：AECC could inhibit the proliferation ，metastasis and invasion of PC 3 cells；the mechanism of which may be associated with regulating the expression of β-catenin signaling pathway related migration and apoptotic factors.
