LINC00519 regulates the proliferation, apoptosis, migration and invasion of gastric cancer cells through the miR-876-3p/HMGA1 axis
DOI：10.3872/j.issn.1007-385x.2021.06.004
- VernacularTitle:LINC00519通过miR-876-3p/HMGA1轴调控胃癌细胞的增殖、凋亡、迁移和侵袭
- Author:
ZHANG Like
1
;
WANG Jianguang
1
;
JING Dongshuai
1
;
LEI Liangliang
1
;
LIU Dechun
1
Author Information
1. Department of Gastrointestinal Surgery, the First Affiliated Hospital of Henan University of Science and Technology; School of Clinical Medicine, Henan University of Science and Technology, Luoyang 471003, Henan, China
- Publication Type:Journal Article
- Keywords:
LINC00519；miR-876-3p；高迁移率蛋白A1；胃癌；增殖；凋亡；迁移；侵袭
- From:
Chinese Journal of Cancer Biotherapy
2021;28(6):574-581
- CountryChina
- Language:Chinese
-
Abstract:
[摘 要] 目的：探讨长基因间非编码RNA 00519（long intergene non-coding RNA 00519，LINC00519）调控miR-876-3p/高迁移率家族蛋白A1（high mobility group protein A1，HMGA1）轴在胃癌HGC-27细胞的增殖、凋亡、迁移和侵袭中的作用。方法：采用qPCR检测胃癌细胞HGC-27和胃黏膜上皮细胞GES-1中LINC00519的表达水平。将HGC-27细胞按转染处理分为si-NC、si-LINC00519、si-LINC00519+anti-miR-NC和si-LINC00519+anti-miR-876-3p组，采用集落形成实验检测细胞克隆形成能力，流式细胞术检测细胞凋亡和周期分布，Transwell实验检测细胞迁移和侵袭。双荧光素酶报告实验和qPCR验证LINC00519与miR-876-3p、miR-876-3p与HMGA1之间的相互作用。结果：HGC-27细胞中LINC00519表达较GES-1细胞显著升高（P<0.05），转染siRNA后si-LINC00519组HGC-27细胞中LINC00519的表达水平较si-NC组显著降低（t=47.294，P<0.01）。与si-NC组比较，si-LINC00519组HGC-27细胞克隆数、迁移侵袭数、S期细胞比例均显著降低（均P<0.01），凋亡率、G0/G1期细胞比例均显著升高（均P<0.01）。与si-LINC00519+anti-miR-NC组比较，si-LINC00519+anti-miR-876-3p组HGC-27细胞克隆数、迁移侵袭数、S期细胞比例升高（均P<0.01），凋亡率、G0/G1期细胞比例显著降低（均P<0.01）。LINC00519能够靶向负调控miR-876-3p的表达，miR-876-3p靶向负调控HMGA1的表达。结论：敲降LINC00519能够通过调控miR-876-3p/HMGA1轴抑制胃癌HGC-27细胞的增殖、迁移和侵袭，诱导细胞凋亡。
- Full text:20210604.pdf
