Study on components with anti-complement activity from ethanol extract of Sanse tablets by UPLC-Q-TOF-MS/MS
10.12206/j.issn.1006-0111.202101036
- VernacularTitle:三色片醇提物抗补体活性物质的UPLC-Q-TOF-MS/MS研究
- Author:
Yan QIN
1
;
Jiajia TAO
1
;
Yun SHEN
1
;
Zhe CHEN
1
;
Yanrong YE
1
Author Information
1. Department of Pharmacy, Zhongshan Hospital, Fudan University, Shanghai
- Keywords:
Sanse tablets;
anti-complement activity;
UPLC-Q-TOF-MS/MS;
chemical components
- From:
Journal of Pharmaceutical Practice
2021;39(4):322-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the chemical components in the ethanol extract of Sanse tablets with anti-complement activity, and to provide scientific basis for its therapeutic effects. Methods The classical anti-complement pathway was used to compare the activity of different portions of Sanse tablet alcohol extract, and to identify the fraction with anti-complement activity. The chemical composition in active fraction was analyzed by UPLC-Q-TOF-MS/MS. The chemical components were identified by comparing the retention time, exact molecular weight and mass spectrum information with the standard chemicals. Results The ethyl acetate fraction of the Sanse tablet ethanol extract had the best anti-complement activity. 42 chemicals were identified, including 16 alkaloids, 15 terpenoids, 6 flavonoids and 5 phenolic acids. Conclusion The characterization of the chemical components in the anti-complement active fraction of Sanse tablets provides a scientific basis for the therapeutic effects of Sanse tablets, which will help the future development of the compound preparations of Chinese medicine in our hospital.
