Effect of diesel exhaust exposure on 8-hydroxy-2' deoxyguanosine level in biological samples of automobile manufacturing workers
10.11763/j.issn.2095-2619.2019.01.008
- Author:
Boya LI
1
;
Xiao JIANG
1
;
Haoyuan TIAN
1
;
Liangying MEI
;
Cheng QI
;
Xin SUN
1
Author Information
1. National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention Beijing 100050, China
- Publication Type:Journal Article
- Keywords:
8-hydroxy-2'-deoxyguanosine;
Ultra-high performance liquid chromatography tandem mass spectrometer;
Oxidative damage;
Biomarker
- From:
China Occupational Medicine
2019;46(01):42-45
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the feasibility of using 8-hydroxy-2'deoxyguanosine(8-OHdG) in blood and urine samples as biomarkers for the evaluation of human DNA oxidative damage caused by diesel exhaust(DE). METHODS: A convenient sampling method was used to select 56 male workers exposed to DE in a car manufacturing factory as exposure group, and 52 male workers without exposure to DE were selected as the control group.Urine samples and blood samples were collected from workers in the 2 groups 8 hours after work, and the levels of 8-OHdG in urine and plasma were measured by ultra-high performance liquid chromatography tandem mass spectrometer. RESULTS: The median level of urinary 8-OHdG in the exposure group was higher than that of control group(2.54 vs 2.03 μg/g Cr, P<0.05). The median levels of plasma 8-OHdG in the exposure group and control group showed no statistical significance(32.20 vs 31.40 ng/L, P>0.05). CONCLUSION: The urinary 8-OHdG can be used as a biomarker for evaluating the oxidative damage induced by DE exposure.
