Sinomenine promotes paired immunoglobulin-like receptor B expression to restrain macrophage classic activation
10.16438/j.0513-4870.2020-1623
- VernacularTitle:青藤碱增加配对免疫球蛋白受体B表达抑制巨噬细胞经典活化
- Author:
Zhi-quan WEI
1
;
Chuan-hong BAO
2
;
Yi-xin CHEN
2
;
Li YAN
1
Author Information
1. Guangxi University Key Laboratory of Basic and Applied Research on Zhuang Medical Prescriptions, Guangxi Traditional Chinese Medicine University, Nanning 530001, China
2. Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi Traditional Chinese Medicine University, Nanning 530200, China
- Publication Type:Research Article
- Keywords:
sinomenine;
rheumatoid arthritis;
paired immunoglobulin-like receptor B;
macrophage classic activation;
M1 macrophage
- From:
Acta Pharmaceutica Sinica
2021;56(6):1644-1652
- CountryChina
- Language:Chinese
-
Abstract:
In this study, in vitro experiments were conducted to investigate that sinomenine inhibits the macrophage classic activation by up-regulating the expression of paired immunoglobulin-like receptor B (PIR-B). A macrophage model with classic activation was established by lipopolysaccharide and interferon-gamma co-stimulation. Real-time fluorescence reverse transcription-polymerase chain reaction was executed for evaluating the PIR-B gene expression, and Western blot for PIR-B protein expression, in macrophages, respectively. The tumor necrosis factor α and interleukin 8 in cell culture supernatant were measured by enzyme-linked immunosorbent assay. The flow cytometry was utilized to detect M1 macrophages. The PIR-B expression in situ was observed by laser scanning confocal microscope. The results showed that sinomenine significantly increased the expression of PIR-B, markedly reduced the percentage of M1 macrophages, and decreased the levels of tumor necrosis factor α and interleukin 8 in the culture supernatant. The above results indicated that sinomenine can significantly inhibit the macrophage classic activation, and its mechanism may be related to the increase of PIR-B expression in macrophages. This pharmacological effect helps explain the pharmacodynamic mechanism of sinomenine in treating rheumatoid arthritis.
