Significance of phosphoinositol metabolism by DNA methylation may contribute in oral leukoplakia carcinogenesis
10.12016/j.issn.2096-1456.2021.10.005
- Author:
WANG Guangchao
1
;
LIU Lijun
1
;
JIANG Weiwen
1
Author Information
1. Department of Oral Mucosal Diseases, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology
- Publication Type:Journal Article
- Keywords:
oral leukoplakia;
oral squamous cell carcinoma;
epigenetics;
DNA methylation;
inositol;
phosphoinositol;
metabolism;
phospholipase C;
inflammation;
calcium signal
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2021;29(10):677-683
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To study the role of DNA methylation in oral leukoplakia carcinogenesis.
Methods: DNA methylation was detected in forty cases of oral squamous cell carcinoma (OSCC), twenty-eight cases of oral leukoplakia (OLK) and forty cases of healthy oral mucosa. Download the expression profile data of OSCC, OLK and healthy oral mucosa from Gene Expression Omnibus (GEO) database. DNA methylation data and expression profile data were compared for repeatability, DNA methylation data for difference analysis and corresponding expression profile data for IPA pathway analysis.
Results :The data analysis showed that DNA methylation had greater flexibility and instability. Ingenuity Pathway Analysis (IPA) analysis showed that genes related to OLK differential methylation sites were mainly concentrated in the process of cell movement and differentiation. Genes related to differential methylation sites of OSCC are mainly enriched in cell proliferation, migration, oxidation regulation, and anti-apoptosis processes. The genes associated with OLK and OSCC differential methylation sites are co-enriched in phosphoinositol metabolism and phospholipase C signaling pathway.
Conclusion:DNA methylation is involved in the formation of oral squamous cell carcinoma, and the activation of phosphoinositol metabolism may promote oral leukinoma.
- Full text:DNA甲基化激活磷酸肌醇代谢在口腔白斑癌变中的意义.pdf
