Expression of NAD(P)H Oxidase Subunits and Their Contribution to Cardiovascular Damage in Aldosterone/Salt-Induced Hypertensive Rat.
10.3346/jkms.2008.23.6.1039
- Author:
Young Mee PARK
1
;
Bong Hee LIM
;
Rhian M TOUYZ
;
Jeong Bae PARK
Author Information
1. Samsung Biomedical Research Institutue, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Aldosterone;
Oxidative Stress;
NAD(P)H Oxidase;
Hypertension
- MeSH:
Acetophenones/administration & dosage;
Aldosterone/administration & dosage/*toxicity;
Aldosterone Antagonists/administration & dosage;
Angiotensin II Type 1 Receptor Blockers/administration & dosage;
Animals;
Anti-Inflammatory Agents, Non-Steroidal/administration & dosage;
Aorta/metabolism/pathology;
Blood Pressure/drug effects;
Hypertension/chemically induced/drug therapy/*enzymology;
Kidney/metabolism/pathology;
Losartan/administration & dosage;
Male;
NADPH Oxidase/antagonists & inhibitors/*metabolism;
Organ Size;
Oxidative Stress;
Protein Subunits/metabolism;
RNA, Messenger/metabolism;
Rats;
Rats, Sprague-Dawley;
Sodium Chloride/administration & dosage;
Spironolactone/administration & dosage;
Superoxides/metabolism
- From:Journal of Korean Medical Science
2008;23(6):1039-1045
- CountryRepublic of Korea
- Language:English
-
Abstract:
NAD(P)H oxidase plays an important role in hypertension and its complication in aldosterone-salt rat. We questioned whether NAD(P)H oxidase subunit expression and activity are modulated by aldosterone and whether this is associated with target- organ damage. Rats were infused with aldosterone (0.75 microgram/hr/day) for 6 weeks and were given 0.9% NaCl+/-losartan (30 mg/kg/day), spironolactone (200 mg/kg/ day), and apocynin (1.5 mM/L). Aldosterone-salt hypertension was prevented completely by spironolactone and modestly by losartan and apocynin. Aldosterone increased aortic NAD(P)H oxidase activity by 34% and spironolactone and losartan inhibited the activity. Aortic expression of the subunits p47(phox), gp91(phox), and p22(phox) increased in aldosterone-infused rats by 5.5, 4.7, and 3.2-fold, respectively, which was decreased completely by spironolactone and partially by losartan and apocynin. Therefore, the increased expression of NAD(P)H oxidase may contribute to cardiovascular damage in aldosterone-salt hypertension through the increased expression of each subunit.
