High-salt exposure induces macrophage polarization to promote proliferation and phenotypic transformation of co-cultured renal fibroblasts.
10.12122/j.issn.1673-4254.2020.10.13
- Author:
Jing LU
1
;
Zhixun BAI
2
;
Xiaoyan KUANG
1
;
Ling LI
1
Author Information
1. Department of Pathology, Zunyi Medical and Pharmaceutical College, Zunyi 563000, China.
2. Department of Nephrology, Second Affiliated Hospital of Zunyi Medical University, Zunyi 563000, China.
- Publication Type:Journal Article
- Keywords:
cell proliferation;
high salt;
macrophages;
phenotypic transformation;
renal fibroblasts
- MeSH:
Cell Proliferation;
Coculture Techniques;
Fibroblasts;
Kidney;
Macrophages;
Transforming Growth Factor beta1/genetics*
- From:
Journal of Southern Medical University
2020;40(10):1472-1479
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate high-salt exposure-induced polarization of mononuclear macrophages and the changes in proliferation and phenotypic transformation of renal fibroblasts in a co-culture system.
METHODS:Cultured mononuclear macrophages were exposed to high salt (161 mmol/L Na +) for 2 h and the surface markers of M0, M1 and M2-type macrophages were detected with RT-qPCR. The culture medium of the macrophages in normal and high-salt groups was collected for detection of the mRNA and protein levels of IL-6 and TGF-β1 using RT-qPCR and ELISA. A co-culture system of high salt-exposed macrophages and renal fibroblasts (NRK-49F) was established using a Transwell chamber, and the changes in proliferation and migration of NRK-49F cells were examined using EdU assay and Transwell assay, respectively. Western blotting was performed to detect the expressions of collagen I, collagen III and collagen α-SMA in NRK-49F cells.
RESULTS:The high salt-exposed macrophages showed significantly increased mRNA levels of M2-type macrophage surface markers mannose receptor and arginase (
CONCLUSIONS:High-salt exposure induces polarization of mononuclear macrophages into M2-type macrophages and promotes secretion of IL-6 and TGF-β1 by the macrophages to induce the proliferation and phenotypic transformation of NRK-49F cells.
