Neuroprotective Effect of Fructus broussonetiae on APP/PS1 Mice via Upregulation of AKT/β-Catenin Signaling.
10.1007/s11655-019-3178-4
- Author:
Ying-Hong LI
1
,
2
;
Yu JIN
3
;
Xu-Sheng WANG
4
;
Xiao-Ling CHEN
5
;
Hong-Bo CHEN
4
;
Ji XU
3
;
Li-Hong DUAN
3
;
Yu-Long WANG
3
;
Xun LUO
6
;
Qing-Mei WANG
7
;
Zheng-Zhi WU
3
Author Information
1. The First Affiliated Hospital of Shenzhen University (the Second People's Hospital of Shenzhen), Shenzhen, Guangdong Province, 518035, China. yinghongli@
2. com.
3. The First Affiliated Hospital of Shenzhen University (the Second People's Hospital of Shenzhen), Shenzhen, Guangdong Province, 518035, China.
4. The Shenzhen Key Laboratory of Health Sciences and Technology, Graduate School at Shenzhen, Tsinghua University, Beijing, 518055, China.
5. Graduate School, Guangzhou Medical University, Guangzhou, Guangdong, 510182, China.
6. Shenzhen Sanming Group, Spaulding Rehabilitation Hospital, Harvard Medical School, Charlestown, Boston, Massachusetts, 02129, USA.
7. Stroke Biological Recovery Laboratory, Spaulding Rehabilitation Hospital, Harvard Medical School, Charlestown, Boston, Massachusetts, 02129, USA.
- Publication Type:Journal Article
- Keywords:
Alzheimer disease;
Chinese medicine;
Fructus broussonetiae;
apoptosis regulatory proteins;
neuroprotective agent
- From:
Chinese journal of integrative medicine
2021;27(2):115-124
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To evaluate the mechanisms underlying the protective effect of Chinese herbal medicine Fructus broussonetiae (FB) in both mouse and cell models of Alzheimer's disease (AD).
METHODS:APP/PS1 mice treated with FB for 2 months and vehicle-treated controls were run through the Morris water maze and object recognition test to evaluate learning and memory capacity. RNA-Seq, Western blotting, and immunofluorescence staining were also conducted to evaluate the effects of FB treatment on various signaling pathways altered in APP/PS1 mice. To further explore the mechanisms underlying FB's protective effect, PC-12 cells were treated with Aβ
RESULTS:FB-treated mice showed improved learning and memory capacity on both the Morris water maze and object recognition tests. RNA-seq of hippocampal tissue from APP/PS1 mice showed that FB had effects on multiple signaling pathways, specifically decreasing cell apoptotic signaling and increasing AKT and β-catenin signaling. Similarly, FB up-regulated both AKT and β-catenin signaling in PC-12 cells pre-treated with Aβ
CONCLUSIONS:FB exerted neuroprotective effects on hippocampal cells of APP/PS1 mice, as well as improved cell viability in an in vitro model of AD. The protective actions of FB occurred via the upregulation of AKT/β-catenin signaling.
